摘要
目的:为了建立“血型糖蛋白A(GlycophorinA,GPA)突变分析”技术。方法:采用杂交瘤技术制备单克隆抗体(McAb)。结果:获得3株分泌抗GPA特异性McAb的杂交瘤细胞系,这3株McAb均属于IgG2a亚类、Kappa型轻链,其中2株特异性抗M型GPA,另外1株特异性抗N型GPA。3株McAb所针对的抗原决定簇位于GPA多肽链N末端第1～39位氨基酸之内,抗体与其结合依赖糖链的完整性。结论:制备的McAb可用于“GPA突变分析”的研究,也可以作为血型试剂在血液免疫学、遗传学和法医学的领域中应用。
Objective: To develop the “glycophorin A (GPA)somatic cell mutation assay”. Methods: Monoclonal antibodies (McAb) were prepared by hybridoma technique . Results: Three mouse monoclonal antibodies against human glycophorin A have been produced . They all belong to IgG2a subclass, Kappa type light chain. Two of them are specific for M form and one for N form GPA. They bind to determinants presented on the 1～39 amino acid N terminal peptide of GPA, their binding depend on intact oligosaccharides. Conclusion: These antibodies is applicable for “GPA somatic cell mutation assay”, also useful in blood immunology, genetics and medical jurisprudence.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2000年第2期73-75,共3页
Chinese Journal of Immunology
基金
全军九五指令性课题!96 0 5 0 0 2
