摘要
目的 为了探讨p16基因与食管癌发生、发展的关系。 方法 应用聚合酶链反应、单链构象多态分析(PCR-SSCP)方法检测30例食管癌中p16基因的纯合性缺失和突变情况,并分析p16基因变异与食管癌的病理关系。 结果 30例食管癌患者中4例为p16基因纯合性缺失,2例存在突变。6例阳性样品与其它样品在年龄、饮酒史方面差异有显著性(P<0.01),与淋巴结转移、病理分期有差异(P<0.05);而与性别、肿瘤长度、肿瘤部位、病理类型无差异(P>0.05)。 结论 抑癌基因p16在食管癌中存在基因变异,其在食管癌的发生。
Objective\ To explore the relationship between p16 gene changes and the tumorigenesis as well as the development of esophageal carcinoma.\ Methods\ Thirty samples were obtained from patients of this institute. The mutations of p16 gene were tested by polymerase chain reaction and single strand conformation polymorphism(PCR SSCP) analysis and the relationships between them and the pathology of esophageal carcinoma were studied.\ Results\ Four homozygous deletions, 2 mutation had been detected. There were extremely significant differences between 6 mutation sample and other samples in age and history of drinking ( P<0.01) , significant differences in lymph node metastasis, and stage of pathology ( P<0.05) . There were no significant differences in sex, length of tumor, location of tumor and type of pathology ( P>0.05) .\ Conclusion\ The mutation of p16 gene occurred in esophageal carcinoma and played an important role in the tumorigenesis and development of esophageal carcinoma.
出处
《中国胸心血管外科临床杂志》
CAS
2000年第1期35-37,共3页
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
关键词
食管癌
P16基因
聚合酶链反应
Esophageal carcinoma\ \ p16 gene\ \ Polymerase chain reaction
