摘要
[目的]探讨不同颜色类型羊草种子对发芽率及愈伤组织诱导率的影响。[方法]根据羊草种子的颜色,将羊草种子分为浅黄色、黄色和黑色3种类型,进行了羊草种子发芽、愈伤组织诱导研究。[结果]浅黄色羊草种子的在各颜色类型羊草种子中发芽率、出愈率较高,发芽率、出愈率分别为32.5%、28.5%。通过TTC活力测定、电导率测定,发现种子活力随着羊草种子颜色的加深而降低。[结论]该研究为建立羊草高频再生体系奠定了基础。
[Objective] To explore the effects of different seed colors on seed germination and callus induction of Leymus chinensis.[Method] According to the color of mature seeds of Leymus chinensis,they were divided three color types,namely,pale yellow,yellow and black.Their seed germination and callus induction were observed.[Result] The pale yellow Leymus chinensis seeds had higher germination rate（32.5%） and rate of callus induction（28.5%）.Based on the results of TTC method and electrical conductivity,the seed viability was decreased with the deepening seed color.[Conclusion] The study laid the foundation for establishing highly efficient plant regeneration system of Leymus chinensis.
出处
《安徽农业科学》
CAS
2012年第3期1501-1503,共3页
Journal of Anhui Agricultural Sciences
基金
国际科技合作项目(2010DFA91930)
黄土高原土壤侵蚀与旱地农业国家重点实验室基金项目(10501-256)
关键词
羊草
种子颜色
生活力
愈伤组织
Leymus chinensis
Seed color
Viability
Callus
