摘要
目的:研究铍离子(Be2+)对口腔链球菌细胞膜元素含量及抗菌性能的影响,探讨镍铬合金修复体对牙周损伤的微生物学机制。方法:含有不同浓度(5mg/L、10mg/L、20mg/L和40mg/L)Be2+的口腔链球菌培养液厌氧培养24h。X线能谱仪分析细胞膜元素含量变化,ABTS-HRP法检测细菌产生H2O2的能力。采用SPSS11.0软件包对数据进行单因素方差分析。结果:口腔链球菌细胞膜钙元素含量减少,钠元素先升高后降低,磷元素升高。Be2+浓度为40mg/L时,口腔链球菌H2O2产量显著下降(P<0.05)。结论:Be2+会改变口腔链球菌细胞膜元素含量,降低H2O2产量,从而导致修复体周围正常微生态环境失衡,引起牙周疾病。
PURPOSE：To evaluate the effect of beryllium（Be2＋） on chemical elements of cell membrane and H2O2 production of Streptococcus oralis（S.oralis）,thus to explore the microbiologic mechanisms of periodontal diseases which may occur after prosthodontic treatment.METHODS： S.oralis was put into artificial saliva with different Be2＋ concentration（5 mg/L,10 mg/L,20 mg/L and 40 mg/L） and anaerobic cultured for 24 hours.The amount of chemical elements in the membrane of S.oralis were tested with X-ray energy dispersive spectroscopy,and the H2O2 produced by S.oralis was detected with ABTS-HRP.The data were analyzed with one-way ANOVA using SPSS 11.0 software package.RESULTS： The amount of calcium in the cell membrane of beryllium treated S.oralis decreased,while the amount of phosphorus increased.Natrium increased with the increase of Be2＋ in the culture,but changed contrarily when S.oralis was treated with 20mg/L Be2＋.H2O2 produced by S.oralis reduced when the concentration of Be2＋ was at 40mg/L（P0.05）.CONCLUSIONS： Beryllium affects the amount of chemical elements in cell membrane and H2O2 production of S.oralis,which may result in a disturbance in the microecologic balance of subgingival microbes after proshodontic treatments and eventually contribute to periodontal diseases.Supported by Science Project of Department of Education of Zhejiang Province（Y200804029） and Science Foundation of Wenzhou Science and Technology Bureau（201150088）.
出处
《上海口腔医学》
CAS
CSCD
2011年第6期572-576,共5页
Shanghai Journal of Stomatology
基金
浙江省教育厅科研项目(Y200804029)
温州市科技局科技计划项目(201150088)
关键词
铍离子
牙科合金
口腔链球菌
细胞膜
口腔微生态
Beryllium
Dental alloy
Streptococcus oralis
Cell membrane
Oral microecology
