摘要
目的是探索通过加载机械振动对骨髓间质干细胞(Mesenchymal stemcells,MSCs)的粘附、增殖和碱性磷酸酶活性的影响。材料和方法将频率(F)为300 Hz,加速度(α)为1.4 gm/s2的垂直的机械振动加载至成骨诱导培养中的MSCs。细胞传代培养后立即加载机械振动,并于1、2、3、6、12、24、48 h后观察细胞贴壁粘附形态变化,并检测培养14 d后细胞碱性磷酸酶(alkaline phosphatase,ALP)活性;对照组不加载机械振动。结果在48小时内实验组可见细胞集落,而在对照组未发现细胞集落的形成;相同时间点实验组的细胞贴壁数量明显多于对照组(P<0.05)。但是两组在第14 d时的碱性磷酸酶水平无明显差异。结论通过加载机械振动促进了细胞粘附,这种方法有可能有用于促进种子细胞与基质材料之间的粘附,从而获得分布均一的组织工程细胞-材料复合物。
Objective The objective if this study was to explore the effects of cells adhesion and the alkaline phosphatase（ALP） activity by loading mechanical vibration on Mesenchymal stem cells（MSCs）.Materials and methods The conditions of the mechanical vibration were that frequency（F） was 300 Hz;the acceleration（α） was 1.4 gm/s2,and the orientation was vertical.The mechanical vibration was loaded immediately on the MSCs that were cultured by osteogenesis induction medium.The morphological study for cell adhesion was performed 1,2,3,6,12,24 and 48 h after subculture,and the alkaline phosphatase（ALP） activity also were detected 14 days after subculture.And the control group was the same as test group only without the vibration.Results there are some cell clonies were found only in test group during the 48 hs after subculture,and there is no cell clone found in control group.The cell number of adhesion were much more than control group（P0.05） at the time points of 1,2,3,6,12,24 and 48 h after subculture（P0.05）.But there was no significant difference of the cell alkaline phosphatase（ALP） activity between the 2 groups at the time point of 14 days.Conclusion The cell adhesion was enhanced by loading the mechanical vibration,and the method may be used for enhancing cell adhesion to the materials for getting a cell-material composite with uniform cell distribution.
出处
《中国实验诊断学》
北大核心
2011年第12期1995-1997,共3页
Chinese Journal of Laboratory Diagnosis
基金
吉林省科技发展计划项目(200705242
20080738)
关键词
机械振动
骨髓间质干细胞
细胞粘附
组织工程
mechanical vibration
mesenchymal stem cells（MSCs）
cell adhesion
tissue engineering
