摘要
目的考察使用壮根灵对党参中党参炔苷含量影响,通过比较说明使用壮根灵对党参品质的影响。方法用高效液相色谱法,色谱柱:ZORBAX Edipse XDB-C18柱(250 mm×4.60 mm5,μm);流动相:乙腈-水溶液(28∶72)(V/V);流速:1.0 mL/min;进样量:10μL;柱温:30℃;检测波长:269 nm。结果 7个不同产地党参中使用壮根灵后党参中党参炔苷含量均明显下降。结论通过SPSS 16.0统计分析,壮根灵对药材中活性成分积累有显著性影响,在党参药材规范化种植过程中应慎重使用壮根灵。
Objective To investigate the influence of plant growth regulator-Zhuanggenling on the content of lobetyolin in crude Dangshen（Radix Codonopsis pilosulae）,and explain the effect of Zhuanggenling on the quality of Dangshen.Methods The HPLC procedure was performed on the chromatographic column of ZORBAX Edipse XDB-C18（250 mm×4.60 mm,5 μm）,and the mobile phase was acetonitrile and water solution（28：72,V/V）.The flow velocity was 1.0 mL/min,sample size,10 μL,temperature,30 ℃ and the detection wavelength,269 nm.Results The content of lobetyolin decreased significantly in Dangshen produced from 7 areas after using Zhuanggenling.Conclusion Zhuanggenling has significant influence on the accumulation of active constituents in Dangshen after SPSS 16.0 statistical analysis,so which should be used carefully in the standardized plantation of Dangshen.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2011年第11期766-768,共3页
Journal of Beijing University of Traditional Chinese Medicine
基金
甘肃省重大科技专项资助项目(No.1002FKDA048)
国家公益性行业科研专项基金资助项目(No.200807020)
关键词
党参
壮根灵
党参炔苷
高效液相
Dangshen（Radix Codonopsis pilosulae）
Zhuanggenling
lobetyolin
HPLC
