摘要
以种内与种间差异以及PCR扩增和测序成功率为评价DNA条形码的重要指标,探索ITS序列作为粒毛盘菌属DNA条形码的可行性。结果表明,ITS成功区分了研究涉及的22个种,其PCR扩增和测序成功率为100%,该片段有望成为该属区分物种的DNA条形码。
The feasibility of ITS as a DNA barcode for the genus Lachnum was investigated.Intra-and inter-specific sequence divergences and easiness of sequence acquisition were considered as important criteria to estimate the barcode marker.The results revealed that ITS showed appropriate intra-and inter-specific variations,was able to separate the investigated 22 species of the genus,and was readily PCR amplified and sequenced.We suggest to use ITS as the potential DNA barcode for the genus.
出处
《菌物学报》
CAS
CSCD
北大核心
2011年第6期932-937,共6页
Mycosystema
基金
Supported by the National Natural Science Foundation of China (No. 31070015)
the Knowledge Innovation Program of the Chinese Academy of Sciences (No. KSCX2-EW-J-6)
