摘要
用DNA体外重组技术,以氧化硫硫杆菌(T.thiooxidans)染色体DNA的EcoPIHindⅢ片段取代pBR322的相应片段,构建成一个重组质粒pSDR12。转化大肠杆菌C600受体菌株后,表现了较强的抗四环素能力。表明了一个专性自养细菌的启动子功能片段在异养细菌中表达。
This paper reports a recombinant plasmid pSDR12 which is constructed through the substitution of the EcoRI-HindⅢ fragment of pBR322 by a specific fragment of chromosomal DNA of T. thiooxidans. After it was transformed into C600, the tranaformants revealed higher levels of Tc resistance. This result shows that a promoter function fragment from autotrophic bacteria is able to express in Escherichia coil.
关键词
氧化硫硫杆菌
启动子
DNA片断
Thiobacillus thiooxidans chromosome, Obligate autotrophic bacteria, te-tracyeline resistance
