摘要
将从长春地区腹泻仔猪盲肠中分离得到的猪三毛滴虫用1640培养基短期培养后,使用碘染色法、瑞氏染色法、姬氏染色法、姬氏-瑞氏复染法、Weigere铁苏木素染色法和鞭毛染色法染色分别对其染色,观察不同虫体形态结构,并比较了不同染色方法的染色效果。结果显示,姬氏染色、瑞氏-姬氏复染和鞭毛染色法对猪三毛滴虫染色效果较好,可获得虫体形态完整、结构清晰的染色标本。
An isolate of Trichomonad suis was isolated from diarrhea pig colon in Changchun.After the isolate was cultured for a short-term,Tritrichomonas suis changchun isolate was stained by six staining methods including the iodine staining,Wright＇s stain,Giemsa stain,Giemsa-Wright staining,Weigert＇s iron hematoxylin staining and flagella staining method.we observed the morphological structure of the isolate and compared the effects of different staining methods.The results showed that Giemsa staining,Wright-Giemsa staining,Giemsa-Wright staining and flagella staining method were better,and could get the stained specimens with complete morphology and clear structure.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2011年第9期1286-1289,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(30970322)
关键词
猪三毛滴虫
染色
比较
Tritrichomonas suis
stain
comparison
