摘要
目的探讨三氧化二砷(As2O3)抑制恶性肿瘤细胞生长及诱导凋亡的生物学效应。方法采用光学显微镜进行形态学观察。溴化二甲噻唑二苯四氮唑(MTT)还原法经浓度为1.25μmol/L、2.50μoml/L、5.00μoml/L、10.00μmol/L、20.00μmol/L As2O3处理后,分别培养48 h、72 h、96 h对胃癌细胞(HGC-27)、肝癌细胞(HepG2)生长的影响。用Hoeschst33258染色后在荧光显微镜下观察细胞凋亡形态。流式细胞仪检测不同浓度As2O3对胃癌细胞周期的影响。结果 HGC-27、HepG2细胞株经As2O3作用后,细胞增殖受到明显抑制;且随着药物浓度的升高及作用时间的延长,抑制率显著升高。经As2O3作用后,Hoechst33258染色可见凋亡细胞的核染色质凝聚且边缘化,并呈现DNA荧光碎片。流式细胞仪检测显示G1/G0细胞由正常对照组的68.55%上升到80.30%,而G2/M期细胞则从11.56%下降至2.53%,出现了明显的G1/G0期阻滞(P<0.05)。结论 As2O3可以明显抑制胃癌细胞的增殖,对肝癌细胞的生长也有一定程度的抑制作用,并有明显的时-效关系和量-效关系,且能将细胞阻滞于G0/G1期;其抗肿瘤的作用机制可能与诱导肝癌细胞和胃癌细胞分化和凋亡、阻滞细胞周期等有关。
Objective To investigate the biological effects of arsenic trioxide （As2O3 ） on human tumor ceils in＂vitro. Methods Human gastric cancer cells （HGC-27） and human hcpatoma cells （HepG2） were treated with different concentrations of As2O3 （ 1.25,2.50,5.00,10.00 and 20.00 （mol/L）. The apoptosis of these cells was detected at the indicated times （48, 72 and 96 hours） and the morphology of apoptosis cells were observed by the fluorescent microscope after staining of HT33258 dye. The change of cell cycle was measured by flow cytometry. Results As2O3 significantly inhibited the proliferation of tumor cells in both dose- and time-dependent manners. After treatment of As2O3 , the nuclear chromatin in apoptotic cells tendered to condensation and marginalization. The data of flow cytometry showed that when treated with As2O3 G0/G1 phase cells increased from 68.55% to 80.30% and G2/M phase cells decreased from 11.56% to 2.53% （P 〈 0.05）. Conclusion As2O3 significantly inhibited the proliferation of tumor cells in both dose- and time- dependent manners. As2O3 arrested most of HGC-27 cells at G1/G0 phase. The mechanism may be associated with the inhibition of cellular growth and proliferation and promotion of apoptosis.
出处
《健康研究》
CAS
2011年第4期254-257,F0003,共5页
Health Research
