摘要
目的探讨喉癌患者外周血组织蛋白酶B(Cathepsin B)、组织蛋白酶L(Cathepsin L)及内源性抑制剂(Stefin A)表达水平的变化与临床诊断和预后的关系。方法收集我院确诊的喉癌患者及正常对照者血清,酶联免疫吸附测定法(Elisa)检测60例喉癌患者及30位正常对照者血清中Cathepsin B、L及Stefin A水平。结果喉癌患者血清中CathepsinB、L水平显著高于正常对照者(P<0.01),二者表达水平呈显著正相关(r=0.434 1,P<0.05);Stefin A水平显著低于正常对照者(P<0.01),但与Cathepsin B、L无显著相关性。结论喉癌患者血清中存在高水平的Cathepsin B、L及低水平的Stefin A,提示检测血清中Cathepsin B、L及Stefin A含量可协助诊断及判断预后。
Objective To study the relation between expression levels of cathepsin B and L as well as stefin A in peripheral blood of patients with laryngeal carcinoma and its diagnosis and prognosis.Methods Cathepsin B and L as well as stefin A levels in serum of 60 laryngeal carcinoma patients and 30 normal controls were measured by enzyme linked immunosorbent assay.Results The levels of cathpsin B and L were significantly higher in serum of laryngeal carcinoma patients than in normal controls（P〈0.01）.The expression levels of cathpsin B and L were positively correlated（r=0.4341,P〈0.05）.The stefin A level was significantly lower in serum of laryngeal carcinoma patients than in normal controls（P〈0.01） but not correlated with the levels of cathpsin B and L.Conclusion High cathpsin B and L level of and low stefin A level in serum of laryngeal carcinoma patients contribute to the diagnosis of laryngeal carcinoma and judgment of its prognosis.
出处
《军医进修学院学报》
CAS
2011年第6期620-621,628,共3页
Academic Journal of Pla Postgraduate Medical School
基金
国家自然科学基金项目(30872853)~~
