摘要
背景:关节置换后深静脉血栓形成的预后在个体间存在很大的差异。有研究表明,巨噬细胞和内皮细胞活性的增高对于血栓溶解管腔再通有促进作用。目的:分析兔深静脉血栓形成后不同时段血浆单核细胞趋化蛋白4及白细胞介素8质量浓度对于反映血栓病情有无特异性。方法:新西兰大白兔78只,随机分为血栓组、假手术组及正常对照组,血栓组造模后14d根据血栓再通情况分为再通组及未通组。分别于血栓后8h,3d,7d,14d抽取耳缘静脉血行单核细胞趋化蛋白4、白细胞介素8质量浓度测定和血常规检测。结果与结论:血栓后第7天血浆单核细胞趋化蛋白4质量浓度再通组较未通组有显著升高(P<0.01),白细胞介素8质量浓度再通组较未通组降低(P<0.05)。其变化与单核细胞、中性粒细胞之间无相关性(P>0.05)。结果表明血栓形成后血浆高质量浓度的单核细胞趋化蛋白4的病例血栓再通情况较好。单核细胞趋化蛋白4的质量浓度通过反映巨噬细胞和内皮细胞的活性,在一定程度上反映了深静脉血栓形成的预后。
BACKGROUND： There are grate individual difference in prognosis of deep venous thrombosis （DVT）. Studies have showed that macrophage and endothelial cell play a great part in recanalization. OBJECTIVE： To study the rabbit plasma monocyte chemoattractant protein 4 （MCP-4） and interleukin-8 （IL-8） levels in different time with Dvr, and to observe whether there is specificity in reflection the thrombus. METHODS： Totally 78 New Zealand rabbits were divided into thrombosis group, sham-surgery group, and normal group. According to the thrombus at 14 days after operation, the thrombosis group was divided into recanalization group and non-recanalization group. The plasma MCP-4 and IL-8 levels were measured and blood routine was performed at 8 hours, 3, 7, and 14 days after operation. RESULTS AND CONCLUSION： Plasma MCP-4 levels were obviously increased in the recanalization group at 7 days after operation （P〈0.01 ）, but the IL-8 levels were decreased compared with the non-recanalization group （P〈0.05）. The variations were not correlated with monocytes or neutrophils （P〉0.05）. It is suggested that there is better recanalization results in thrombus that with higher plasma MCP-4. MCP-4 levels can forecast thrombosis prognosis via reflecting activity of macrophage and endothelial cells.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第11期2013-2017,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
