摘要
目的探讨不同光照剂量的光动力疗法(PDT)对小鼠Heps肝癌移植瘤的抗肿瘤作用及局部免疫效应。方法 69只ICR小鼠皮下接种Heps肝癌细胞建立荷瘤鼠模型,随机分为对照组、光照低剂量PDT组(能量密度135 J/cm2)和光照高剂量PDT组(能量密度215～225 J/cm2)。治疗后定期测量各组肿瘤体积,计算抑瘤率。观察HE染色的瘤组织病理学改变,免疫组化法检测肿瘤组织的CD8+细胞毒性T淋巴细胞(CD8+CTLs)。结果 (1)光照低剂量PDT组和光照高剂量PDT组抑瘤率分别为73.9%、96.3%;光照高剂量PDT组的抑瘤效果显著高于光照低剂量PDT组(P<0.01)。(2)PDT后2、24和72 h肿瘤组织先后出现肿瘤细胞空泡样变性、广泛核固缩及凝固性坏死;肿瘤中微血管淤血扩张、出血和管壁破坏,上述病理改变光照高剂量PDT组更为显著。(3)治疗后72 h光照低剂量PDT组和光照高剂量PDT组肿瘤中CD8+CTLs数量均明显高于对照组(P<0.01,P<0.05);而光照低剂量PDT组肿瘤中CD8+CTLs数量又显著高于光照高剂量PDT组(P<0.05)。结论光照高剂量PDT组的抑瘤效果显著高于光照低能剂量PDT组;PDT可引起细胞毒性T淋巴细胞在肿瘤局部浸润增加,光照低剂量PDT组对肿瘤局部CD8+CTLs的免疫增强效应优于光照高剂量PDT组。
Objective To investigate the anti-tumor and local immune efficacy of photodynamic therapy（PDT） with different light dose on mice Heps transplanted hepatoma. Methods The hepatoma model was established by subcutaneous inoculation with Heps cells in sixty-nine ICR mice,who were then divided into control group,lower light dose PDT group（energy density 135 J/cm2） and high light dose PDT group（energy density 215～225 J/cm2）.The tumor sizes were measured regularly after PDT,and the tumor suppressive rate was calculated.Pathological sections of tumorous tissues were observed by HE staining.The number of CD8 ＋ cytotoxic T lymphocytes（CTLs） infiltrated into the tumor was detected by immunohistochemical staining. Results（1）The tumor inhibited rate in lower light dose PDT group and high light dose group was 73.9% and 96.3%,respectively.The tumor inhibited effect in high light dose PDT group was significantly higher than the lower one（P 0.01）.（2）At various time points after PDT,tumor cells displayed gradually vacuolated degeneration,extensive cells nuclear pyknosis leading to coagulation necrosis.The progressive changes were seen in the tumor microvessels at the three time points after PDT,including congestion,dilatation,hemorrhage and angiolysis.In high light dose PDT group,the pathological changes in tumorous tissues were more remarkable.（3）Compared with control group,the number of CD8 ＋ CTLs infiltrated into tumors was increased significantly（P 0.01,P 0.05） at 72 h after treatment in both lower and high light dose PDT groups,respectively.The number of CD8 ＋ CTLs infiltrated in lower light dose PDT group was significantly higher than the high one. Conclusions The tumor inhibited effect in high light dose PDT group is significantly higher than the lower one.PDT results in increase of the numbers of CD8＋CTLs infiltrated into the tumors.The improved immune efficacy on CD8 ＋ CTLs infiltrated into tumors in lower light dose PDT group is higher than that in the high one.
出处
《中国激光医学杂志》
CAS
CSCD
2011年第2期69-73,129,共6页
Chinese Journal of Laser Medicine & Surgery
基金
南京军区"十一五"医药卫生科研基金重点课题资助(项目编号:06Z18)
