摘要
应用RAPD技术对吐鲁番地区火焰山及艾丁湖区域分离的15株土壤绿藻(chlorophyta)品系的遗传多样性及其亲缘关系进行探讨。结果表明:从20个随机引物中,筛选出多态性和重复性较好且谱带清晰的引物8个,这8个引物扩增出的DNA片段大多在300～2 000 bp之间,所形成的多态性位点数差距较大,显示该区域土壤绿藻具有较丰富的遗传多样性;15株土壤绿藻扩增共得到74条谱带,71条多态性带,其多态性比率为95.95%;聚类分析显示15株土壤绿藻明显地聚为2大类,与其来源相对应,即隶属于同一亚组或相近亚组的不同种基本归为一类,其种间关系与传统的形态学分类结果相吻合。
The random amplified polymorphic DNA（RAPD） technology was used to estimate the genetic diversity and relationships among 15 strains of desert soil green algae isolated from the area of Ayding Lake and Yalkun Mountain in Turpan of Xinjiang Uyghur Autonomous Region.8 primers that produced polymorphic and reproducible clear bands were screened out from 20 arbitrary primers.The size of the DNA fragments amplified by these primers mainly ranged from 300 to 2000bp and formed various RAPD fingerprints with different band patterns and sizes.The results showed that the desert soil green algae we studied had a relative high level genetic diversity whith 71 polymorphic bands among total 74 and the percentage of polymorphic loci was 95.95%.A dendrogram based on genetic distances using UPGMA method showed that the desert soil green algae were clearly divided into two groups which corresponds to its origin,namely the strain relationships and traditional classification result.
出处
《西北植物学报》
CAS
CSCD
北大核心
2011年第4期659-664,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(30860119)
新疆大学植物生物技术重点实验室项目
关键词
荒漠土壤绿藻
RAPD
遗传多样性
遗传距离
亲缘关系
desert soil green algae
RAPD
genetic diversity
genetic distance
phylogenetic relationship
