摘要
用流式细胞仪、 Ｈｏｅｃｈｓｔ３３２５８ 荧光素核染后显微镜下计数和电镜三种方法观察 Ｌ Ａ Ｋ 细胞培养上清和裂解液诱导 Ｒａｊｉ肿瘤细胞的凋亡。发现该上清和裂解液与靶细胞共同作用３ｈ 后瘤细胞凋亡率显著增高， 到９ｈ 达最高点， 亚二倍体 Ｄ Ｎ Ａ峰积分面积可达４８ ％ 。在电镜下观察到胞体缩小、表面平滑化、染色质靠边浓集、不连续凋亡小体形成等典型的细胞凋亡现象。提示诱导肿瘤细胞的凋亡是 Ｌ Ａ Ｋ 细胞杀伤机制之一， 并可能通过可溶性因子发挥作用。
further investigate the tumor cell killing mechanism by LAK cells Apoptosis of Raji cell induced by culture supernatant and freeze thawed fluid of LAK cell were observed and detected by fluo rescence microscopy,electron microscopy and flow cytometry analysis techniques Raji cells treated with LAK cell culture supernatant and its freeze thawed fluid underwent the apoptosis process which persisted throughout 15 hours at least,and displayed a time dependent relationship The phenomena appeared from 3 hours after treatment,and reach the peak at 9 hour point These results indicated that the apoptosis in duced by LAK cells via soluble factor might be an important mechanism of LAK cell mediated cytotoxicity
出处
《上海免疫学杂志》
CSCD
北大核心
1999年第4期210-212,共3页
Shanghai Journal of Immunology
