摘要
目的 探讨汉族人载脂蛋白 Ｂ（ａｐｏｌｉｐｏｐｒｏｔｅｉｎ Ｂ， Ａｐｏ Ｂ） 基因变异与动脉粥样硬化性脑梗死的关系。方法 用聚合酶链反应（ Ｐ Ｃ Ｒ） 对１５０ 例动脉粥样硬化性脑梗死患者及３０１ 名健康人的 Ａｐｏ Ｂ 基因 Ｘｂａ Ｉ酶切位点限制性片段长度多态性进行了研究。结果 脑梗死患者及正常人均以 Ｘ－ Ｘ－ 为主要基因型，频率分别为０９０７ 、０９４８ ，少见 Ｘ＋ Ｘ－ 、未见 Ｘ＋ Ｘ＋ 基因型，２ 组人群基因型分布处于 Ｈａｒｄｙ Ｗｅｉｎｂｅｒｇ 平衡；少见的 Ｘ＋ 等位基因， 其频率明显低于文献报道的白种人，其频率分别为００２７ 、０４１８ 、０４５４ 、０４７９（ 均为 Ｐ＜ ００１） ；脑梗死组 Ｘ＋ 等位基因频率明显高于对照组，分别为００５３ 、００２７（ Ｐ＜ ００５） ；脑梗死组平均血清总胆固醇水平明显升高，分别为（５３ ±１３） 、（４９ ±１３） ｍｍｏｌ／ Ｌ（ Ｐ＜ ００５） ， Ａｐｏ Ａ Ｉ水平、 Ａｐｏ Ａ Ｉ／ Ａｐｏ Ｂ 比值均明显降低，分别为（１０２ ±０３４） 、（１２６ ±０４０） ｇ／ Ｌ，（ 均为 Ｐ＜ ００１） ；脑梗死组具有 Ｘ＋ Ｘ－ 基因型个体血清 Ａｐｏ Ｂ
Objective To explore the association of variance at XbaI site of apolipoprotein B (ApoB ) gene and atherosclerotic cerebral infarction (ACI) in Chinese Han population Methods Methods Using polymerase chain reaction (PCR) techniques, we studied the restrict fragment length polymorphism (RFLP) at XbaI site of ApoB gene in 150 patients with ACI and 301 healthy age, sex matched individuals from a population of Chinese Han nationality in Beijing Results In both ACI group and control group, X-X- genotype was the most frequent one (frequency: 0 907, 0 948) and we did not find X+X+ genotype. The distribution of genotypes in the two groups was at the Hardy Weiberg equilibriums The frequency of rare allele X+ was significantly lower in Chinese Han than that reported in Caucasians (0 027 vs 0 418, 0 454, 0 479, P <0 01) The higher frequency of rare allele X+ was found in the ACI group as compared with the control group (0 053 vs 0 027, P <0 05) markedly increased levels of TC (5 3±1 3)、(4 9±1 3) mmol/L ( P <0 05 ),and low levels of Apo AI, Apo AI/ ApoB in the ACI group were observed(1 02±0 34)、 (1 26±0 40) g/L,( P <0 01); X+X- genotype was associated with higher levels of ApoB compared with the levels of X-X- genotype in the ACI group (0 89±0 29)g/L、 (0 78±0 17) g/L ( P < 0 05) Conclusion X+ allele of ApoB gene may be associated with ACI to some extent in the Chinese population, and presumbly through its effect on ApoB metabolism it increases the susceptibility to ACI
出处
《中华医学杂志》
CAS
CSCD
北大核心
1999年第8期603-606,共4页
National Medical Journal of China
基金
北京市自然科学基金
