摘要
目的研究新西兰大白兔骨髓来源内皮祖细胞(EPC)的分离培养和鉴定方法,证实骨髓是获得内皮祖细胞的理想来源之一,为后续研究提供材料准备。方法取兔骨髓,密度梯度离心法分离单个核细胞(MNC),置于DMEM-L培养基中培养,动态观察细胞生长过程,采用免疫荧光染色和细胞荧光化学法鉴定培养的细胞。结果培养10 d的EPC能吞噬ac-LDL并与凝集素UEA-1相结合,同时表达CD34和CD31相关抗原。结论密度梯度离心法从兔骨髓中分离的MNC在一定条件下,能够分化为具有内皮祖细胞特征的细胞,为后续的实验研究提供了细胞来源。
Objective To investigate how to isolate, culture and identify the endothelial progenitor cells from rabbit bone marrow in order to get ready for subsequent experiments. Methods EPCs were obtained from in vitro culture of MNCs which were isolated with density gradient centrifugation from rabbit bone marrow. Process of cell growth was observed. Cultivated cells were identified by immunofluorescence and cell chemical fluorescence detection. Results After 10 days cultured,EPCs were double positive for Dil- acLDL uptaking and FITC-UEA-1 binding and expressing CD34 and CD31 related antigen. Conclusion The .mononuclear cells isolated from rabbit bone marrow by gradient centrifugation can be induced into endothelial progenitor cells in vitro. The system of cell culture can be applied to subsequent experiments in cell transplantation.
出处
《重庆医学》
CAS
CSCD
北大核心
2011年第10期952-953,F0002,F0003,共4页
Chongqing medicine
基金
第三军医大学大坪医院国家重点实验室开放基金(SKLKF200922)
关键词
骨髓
内皮祖细胞
分离培养
bone marrow
endothelial progenitor cells
cell isolation
