摘要
目的：研究活动性巨细胞病毒（ＨＣＭＶ）感染孕妇红细胞免疫功能和ＮＫ细胞活性，观察特异性转移因子（ＳＴＦ）和非特异性转移因子（ＮＳＴＦ）的治疗效果。方法：采用酶联免疫吸附试验（ＥＬＩＳＡ）检测ＨＣＭＶ 特异性ＩｇＭ 和ＩｇＡ，用免疫粘附试验检测红细胞免疫粘附（ＲＣＩＡ）功能及调节因子，用ＭＴＴ比色法测定ＮＫ细胞活性，分别给予３８ 例ＨＣＭＶ 感染孕妇ＳＴＦ和ＮＳＴＦ治疗。结果：ＨＣＭＶ 感染组的ＲＣＩＡ 是１６．６３％ ±２．９６％ ，明显低于非感染组的１８．６９％ ±３．２８％ 和未孕组的２０．６８％ ±２．９５％ ；感染组的ＮＫ细胞活性为４２．７９％ ±１１．９８％ ，也显著低于非感染组的４８．７２％ ±１１．３２％ 和未孕组的５５．４６％ ±１２．４７％ ；感染组的红细胞免疫粘附抑制率（ＲＦＩＲ）为２１．１０％ ±４．４６％ ，明显高于非感染组的１８．７３％ ±４．６７％ 和未孕组的１６．７３％ ±３．８３％ ；不同孕期感染孕妇的各项免疫指标差异无显著性；免疫治疗观察，ＳＴＦ的转阴率为６３．２％ ，明显高于ＮＳＴＦ的２６．３％ ，两种转移因子均能使ＮＫ细胞活性增高。结论：ＨＣＭＶ感染孕妇的免疫功能降低，转移因子治疗可以提高ＮＫ细?
Objective:To study erythrocyte immune function and NK cell activity of pregnant woman with active human cytomegalovirus(HCMV) infection,and therapeutic effect of specific transfer factor(STF) and non specific transfer factor(NSTF)on them.Methods:Specific IgM and IgA of HCMV was determined by enzyme linked immunosorbent assay(ELISA).Immune adherence test was for studying red cell immune adherence(RCIA)function and regulatory factor.MTT colorimetric method was used to determine NK cell activity.STF and NSTF were separately given to 38 pregnant woman with HCMV infection. Results: RCIA in HCMV infection group(16.63%±2.96%)was obviously lower than that of non infection group(18.69%±3.28%)and non pregnancy group(20.68%± 2.95 %).NK cell activity in the infection group(42.79%±11.98%)was also significantly lower than that of non infection group(48.72%±11.32%)and non pregnancy group( 55.46 %±12.47%).Rosette forming inhibitory rate (RFIR)in the infection group(21.10%±4.46%)was significantly higher than that of non infection group(18.73%±4.67%)and non pregnancy group(16.73%±3.83%).No significance was found between immune indexes of the infection group at various pregnant period.The rate of turning to negativity(63.2%)in STF group was significantly higher than that in NSTF group (26.3%).NK cell activity was enhanced in the infection group with two transfer factors therapy.Conclusion: Immune function of pregnant woman with active HCMV infection is decreased,and transfer factors therapy can increase NK cell activity and make HCMV IgM or IgA negative in serum.
出处
《现代妇产科进展》
CSCD
1999年第3期234-237,共4页
Progress in Obstetrics and Gynecology
