摘要
目的:观察甲基强的松龙(methylprednisolone,MP)对急性脊髓损伤后下丘脑室旁核(paraventricular nucleus,PVN)神经元FOS和一氧化氮合酶(nitric oxide synthase,NOS)表达的影响。方法:将大鼠分为对照组、脊髓损伤组、MP治疗组。脊髓损伤组在第4胸髓节段横断;治疗组在脊髓横断后每天给予1次静脉注射甲基强的松龙(30 mg/kg),分别持续1天、1周或4周。NADPH-d组织化学及FOS免疫组织化学方法观察各组PVN内FOS与NOS表达情况。结果:与对照组相比,脊髓损伤组PVN内FOS、NOS表达显著增高。MP治疗1周和4周PVN内FOS、NOS表达较损伤组显著下降(P<0.01)。结论:甲基强的松龙可能对脊髓损伤后PVN内神经元继发性损伤有一定保护作用。
Objective： To observe the effects of methylprednisolone（MP） on expressions of FOS and NOS in the paraventricular nucleus（PVN） of the hypothalamusfollowing the acute spinal cord injury（SCI）.Methods：The total of 36 adult SD rats were randomly divided into 3 groups： control group,SCI group and MP treatment group.The rats in SCI and MP treatment groups were received the spinal transection at the fourth thoracic segment（T4）.MP treated rats were given intravenous injection MP（30 mg/kg） for once,or once daily for one week or four weeks,respectively.FOS and/or NOS positive neurons were mapped in the PVN using NADPH-d histochemistry combined with Fos immunohistochemistry.Results：Compared with control group,the numbers of FOS positive neurons and NOS positive neurons were increased in the PVN,which were decreased following MP treatment for one week or four weeks.Conclusion：MP may have the protective effects on neurons in the PVN following the SCI.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第2期190-192,259,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
淮安市科技发展基金项目(HAS07050)
关键词
甲基强的松龙
脊髓损伤
室旁核
FOS
NOS
methylprednisolone（MP） spinal cord injury paraventricular nucleus（PVN） FOS NOS
