摘要
对用ICCD作为HPLC的LIF检测器进行了研究,建立了一种具有波谱分辨功能的HPLC-LIF-ICCD检测系统,考察了仪器光路、激光功率、ICCD操作参数等多种因素对其性能的影响.在优化条件下,对异硫氰酸荧光素的浓度检测限为2.5×10^(-13)mol/L,质量检测限达到2.5×10^(-18)mol.
A high performance liquid chromatography-laser induced fluorescence-intensified charge coupled device system was estabKshed using commercially available intensified charge coupled device (CCD) and several factors influencing on its performance were investigated in detail. The system wasshowed to be suitable for single or multiwavelength detection. Its mass detection limit is 2.5×10-18 molusing fluorescein isothiocyanate as probe.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1999年第10期1231-1236,共6页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金
中国科学院九五重大项目资助课题
关键词
高效液相色谱
激光诱导荧光
ICCD
检测器
High performance liquid chromatography, laser induced fluorescence, intensified charge coupled device, separation
