摘要
目的:研究贵州世居少数民族-黔西、威宁彝族人群IL-10基因启动子区-819位点(IL10-819)和趋化因子受体5(chemokine receptor 5,CCR5)基因启动子区-59029位点(CCR5-59029)与HBV易感性的相关性.方法:对研究人群采用序列特异性引物-聚合酶链式反应(PCR-SSP)方法分析IL-10基因启动子区-819位点多态性和限制性片段长度多态性-聚合酶链式反应(PCR-RFLP)方法分析CCR5基因启动子区-59029位点.结果:IL10-819基因型频率在贵州黔西彝族感染组与非感染组中的分布差异有统计学意义(P<0.05),在贵州威宁彝族感染组与非感染组中的分布差异无统计学意义;CCR5-59029基因威宁彝族感染组与非感染组中的分布差异有统计学意义(P<0.05),在贵州黔西彝族感染组与非感染组中的分布差异无统计学意义.结论:IL10-819基因和CCR5-59029基因多态性与彝族乙肝感染情况可能存在相关性,IL10-819基因和CCR5-59029基因多态性可能是乙肝易感性的因素之一.
AIM：To investigate the distribution of chemokine receptor 5 gene 59029A/G（CCR5-59029） and interleukin 10 819 T/C（IL10-819） polymorphisms in two Yi minority populations in Guizhou Province,China,and to analyze their association with HBV infection.METHODS：Blood samples were taken from 105 and 108 Yi ethic individuals in Qianxi and Weining,respectively.The CCR5-59029 polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）,while the IL10-819 polymorphism was genotyped by polymerase chain reaction-sequence-specific primer（PCR-SSP）.DNA sequencing was performed to verify the above results.RESULTS：The genotype frequency of IL10-819 polymorphism differed significantly between HBV-infected subjects and non-infected ones in the Qianxi Yi minority population（P〈0.05）,but showed no statistical difference in the Weining Yi minority population（P〈0.05）.In contrast,the genotype frequency of CCR5-59029 differed significantly between HBV-infected subjects and non-infected ones in the Weining Yi minority population（P〈0.05）,but showed no statistical difference in the Qianxi Yi minority population（P〈0.05）.CONCLUSION：CCR5-59029 and IL10-819 polymorphisms are associated with susceptibility to HBV infection in the Yi minority population in Guizhou Province,China.
出处
《世界华人消化杂志》
CAS
北大核心
2010年第31期3372-3377,共6页
World Chinese Journal of Digestology
基金
贵州省科技厅科技计划课题基金资助项目
No.黔科合SY字[2008]3053
No.黔科合SY字[2010]3001
贵州省卫生厅基金资助项目
No.黔卫发[2009]122~~
