摘要
以荔枝和龙眼部分种质幼叶为材料,采用等体积饱和对二氯苯和2mmol·L-18–羟基喹啉混合水溶液预处理,卡诺固定液固定,酸解与酶解相结合,改良卡宝品红染色,观察了体细胞染色体数。结果表明,‘裂叶’龙眼为单倍体(2n=x=15),且生长表现良好;用以创建荔枝遗传图谱的作图群体母本‘马贵荔’和父本‘焦核三月红’及用以构建龙眼遗传图谱的两个作图群体母本‘凤梨朵’和‘石硖’,父本‘大乌圆’和‘中秋一号’均为二倍体(2n=2x=30),通过远缘杂交得到的2株荔枝和龙眼的属间杂种A和B也为二倍体,且生长良好;一些性状独特的荔枝,如‘紫娘喜’、‘无核荔’、‘MS-07’、‘SGA’、‘YA1’、‘YN4’,以及龙眼‘双季’也均为二倍体。
Young leaves of several rare germlasm resources in litchi and longan were used to observe chromosome numbers and identify their ploidy after pretreated with the mixture of 1︰1 aqueous solution of saturated paradichlorobenzene and 2 mmol · L-1 8-hydroxyquinoline,fixed by Carnoy’s fixative, hydrolyzed with HCl and enzymes,and stained in improved Carbol-fuchsin. The results showed that ‘Lieye’longan was a haploid（2n = x = 15）and grew normally. The parents used to create three mapping groups,including litchi parent ‘Maguili’ and paternal ‘Jiaohesanyuehong’,and longan paren‘tFengliduo’, ‘Shixia’and paternal‘Dawuyuan’,‘Zhongqiu 1’were all diploids（2n = 2x = 30）. The intergeneric hybrid（sA and B）between Litchi and Dimocarpus were also diploids and could grow normally. Moreover, several germplasm resources with distinct trait including litch‘iZiniangxi’,‘Wuheli’,‘MS-07’,‘SGA’, ‘YA1’and‘YN4’as well as a longan‘Shuangji’were all diploids.
出处
《园艺学报》
CAS
CSCD
北大核心
2010年第12期1991-1994,共4页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(30270922
30771498)
国家自然科学基金国际(地区)合作交流项目(30510103125)
国家科技支撑计划子课题(5300-A07050)
中国博士后科学基金项目(20070410837
20080440763)
广东省国际合作项目(2009B050300004)
广东省科技计划项目(2009B020200010)
华南农业大学国际合作交流基金项目(2007K055)
现代农业产业技术体系建设专项项目
关键词
荔枝
龙眼
幼叶
染色体
单倍体
litchi
longan
young leaf
chromosome
haploid
