摘要
乌索酸是一种常见的五环三萜类化合物,本文考察了其对原单核白血病细胞株THP-1的分化诱导作用。乌索酸作用72h后,通过显微镜形态观察,部分THP-1细胞贴壁生长,形态具有向巨噬细胞分化的特征。结晶紫染色结果显示细胞贴壁程度呈剂量依赖型增长。利用流式细胞分析技术分化标志物,发现CD11b和CD14阳性细胞百分比均有显著上调。Western blotting结果表明乌索酸能上调分化相关蛋白C/EBPα的p42亚基并且下调分化负调控因子c-myc的蛋白水平,且对二者的表达调控作用具有浓度和时间相关性。因此,乌索酸能够诱导THP-1细胞向单核/巨噬细胞方向分化。其作用机制可能是上调C/EBPα的p42亚基,进而下调c-myc,从而达到分化诱导作用。
Ursolic acid（UA）,a pentacyclic triterpenoid compound,is widely distributed in the plant kingdom.The present work was performed to investigate the potential role of UA in the differentiation of human monocytic leukemia cell line THP-1 as well as the underlying mechanisms for it.After 72 h treatment with UA,morphology of THP-1 cells was observed by phase contrast microscope.Some of the cells were adherent and turned into macrophage-like cells.Moreover,the adhesion rate was enhanced in a dose-dependent manner,detected by crystal violet staining assay.In addition,the differentiation markers,CD11b and CD14,the positive cells were significantly increased after the treatment with UA analyzed by flow cytometry.Finally,western blotting showed that UA up-regulated p42 C/EBPα protein,while inhibiting the expression of c-myc,both in a concentration-and time-dependent manner.Taken together,ursolic acid can induce THP-1 cells differentiation into monocyte/macrophage lineage probably through the regulation of p42 C/EBPα and c-myc.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2010年第6期572-576,共5页
Journal of China Pharmaceutical University
