摘要
目的:探讨胰腺癌组织中E-钙粘素(E-cadherin)的表达与胰腺癌临床病理特征及预后的关系,及E-cadherin在胰腺癌血管生成中的作用。方法:采用免疫组化Power Vision法对49例胰腺癌组织及癌旁正常胰腺组织的E-cadherin、血管内皮生长因子(Vascular endothelial growth factor,VEGF)和CD34抗体的表达情况进行检测。结果:胰腺癌组织中E-cadherin阳性表达阳性率为73.5%,癌旁正常胰腺组织阳性率仅为16.3%,两者比较差异有统计学意义(P<0.05)。E-cadherin蛋白和VEGF蛋白表达呈正相关(P<0.05);E-cadherin阳性组的微血管密度(Microvessel density,MVD)值明显高于阴性低表达组(P<0.05)。E-cadherin的表达与胰腺癌组织的分化程度、浸润程度、血管侵袭、淋巴结转移及pTNM分期呈明显相关(P<0.05)。E-cadherin阳性表达组患者的中位生存时间(18.00±3.65)月低于阴性表达组(30.00±8.01)月,阳性表达组的预后显著差于阴性表达组,差异有统计学意义(P<0.05)。结论:E-cadherin的异常表达可作为评价胰腺癌预后的辅助指标。E-cadherin可能激活VEGF通道,从而诱导胰腺癌血管的生成。
Objective：Toinvestigate the association ofE-cadherin expression with the clinicopathologic features,prognosis and angiogenesis in pancreatic carcinoma. Methods：Immunohistochemistry（Power Vision method）were performed to investigate the expression of E-cadherin protein,VEGF protein and CD34 in 49 patients with pancreatic carcinoma. Results：Reduced or loss of membranous expression and cytoplasmic localization of E-cadherin protein were found in pancreatic cancer tissues （36/49,73.5%）by using immunohistochemistry. Moreover there was an positive relationship among E-cadherin protein ,VEGF protein and microvessel density expression （P0.05）in pancreatic cancer tissues. Reduced expression of E-cadherin occurred more frequently in poor differentiation （χ 2 = 8.279,P0.05）,positive lymph metastasis（χ 2 =4.045,P0.05）,positive vascular invasion（χ 2 = 4.738,P0.05）,deeper invasion（χ 2 = 8.653,P0.05）and advanced stage （χ 2 = 11.006,P 0.05）.Additionally,reduced expression of E-cadherin protein in tumor correlated with a worse prognosis and normal expression with a better survival rate（P=0.023）.Conclusion：The altered expression of E-cadherin can be regarded as an assistant marker in evaluating the prognosis of pancreatic carcinoma. E-cadherin may activate VEGF-channels,and induce the angiogenesis of pancreatic carcinoma.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2010年第10期1502-1505,共4页
Journal of Chongqing Medical University
