摘要
目的化学去细胞异体神经具有与正常神经相似的物理和生化特性,是周围神经缺损修复的较好神经移植替代物。进一步探讨化学去细胞异体神经的促神经趋化性再生作用。方法健康成年SD大鼠5只,雌雄不限,体重270～300g,用于制备化学去细胞异体神经;健康成年Wistar大鼠18只,雌雄不限,体重300～320g,用于神经移植动物实验。将Wistar大鼠随机分为实验组和对照组(n=9),制备大鼠左侧坐骨神经缺损(10mm)模型,实验组以化学去细胞异体神经与神经断端吻合,对照组将自体神经近、远端翻转后吻合。神经移植后3个月,各组3只实验动物取腓肠神经行乙酰胆碱酯酶和碳酸酐酶染色观察;各组其余6只实验动物行脊髓背根神经节切除术,术后3周取腓肠神经行病理染色,病理切片行神经纤维计数,并计算错接率。结果乙酰胆碱酯酶及碳酸酐酶染色观察显示,实验组腓肠神经乙酰胆碱酯酶染色棕色颗粒较对照组少,碳酸酐酶染色黑色颗粒较对照组多。脊髓背根神经节切除定量评价:实验组神经纤维计数为4257±285,对照组为4494±310;实验组及对照组腓肠神经运动纤维错接率分别为2.27%±0.28%和7.65%±0.68%,差异有统计学意义(P<0.05)。结论化学去细胞异体神经移植物不仅在桥接修复神经缺损中起到支架、支撑作用,还具有明显的促神经趋化性再生作用。
Objective To investigate the promotion effect of neurotropic reinnervation with chemically extracted acellular nerve allograft. Methods The sciatic nerves of 5 healthy adult SD rats,regardless of gender and weighing 270-300 g,were collected to prepare chemically extracted acellular nerve allograft.Eighteen healthy adult Wistar rats,regardless of gender and weighing 300-320g,were made the model of left sciatic nerve defect（10 mm） and randomly divided into 2 groups：autograft（control group,n=9） and allograft group（experimental group,n=9）.The defects were bridged by acellular nerve allograft in experimental group and by autograft by turning over the proximal and distal ends of the nerve in control group.At 3 months after transplantation,dorsal root ganglion（DRG） resection operation was performed in 6 rats of 2 groups.At 3 weeks after operation,the sural nerves were harvested to calculate the misdirection rate of nerve fibers with pathological staining and compute-assisted image analysis.Cholinesterase staining and carbonic anhydrase staining were performed in the sural nerve of 3 rats that did not undergo DRG resection at 3 months. Results The results of cholinesterase staining and carbonic anhydrase staining showed that experimental group had less brown granules and more black granules than control group.After DRG resection,count of myelinated nerve fiber were 4 257 ± 285 in the experimental group and 4 494 ± 310 in the control group significant（P 0.05）.The misdirection rate was 2.27% ± 0.28% and 7.65% ± 0.68% in the experimental group and in the control group respectively,showing significant difference（P 0.05）. Conclusion Chemically extracted acellular nerve allograft can not only act as a scaffold in the period of nerve defects repair,but markedly enhance the effects of neurotropic reinnervation.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2010年第11期1288-1292,共5页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家自然科学基金资助项目(30571875)
北京市自然科学基金重点资助项目(03G502)~~
关键词
化学去细胞异体神经
周围神经缺损
神经再生
神经趋化性
Chemically extracted acellular nerve allograft Peripheral nerve defect Nerve regeneration Neurotropism
