摘要
本文采用逆转录－多聚酶链反应（ＲＴ－ＰＣＲ）扩增了汉坦病毒陈株的Ｓ基因的编码区序列，克隆入真核表达载体ＰＣＭＶ４，构建了可表达汉坦病毒Ｓ基因的真核表达载体ＰＣＭＶ４－ＣｈｅｎＳ１．３。用电穿孔法转染ＣＯＳ－７细胞，免疫荧光和ＥＬＩＳＡ检测表明，目的基因在ＣＯＳ－７细胞中获得瞬时表达。
We amplified the coding region of S gene of HTNV strain Chen by RT PCR and this fragment was cloned into eukaryotic expression vector PCMV 4 The recombinant plasmid was transfected into COS 7 cell by electroporation Immunoflourescence and ELISA assay showed that the recombinant NP was transient expressed in COS 7 cell
出处
《中国人兽共患病杂志》
CSCD
北大核心
1999年第2期9-11,共3页
Chinese Journal of Zoonoses
基金
国家自然科学基金
军队杰出人才基金
