摘要
采用扩增片段长度多态性（AFLP）分子标记技术,利用筛选出的6对多态性较强的引物组合（GAA/CAC、GAA/CAG、GAA/CTT、GAG/CTC、GAT/CTC、GAT/CTT）对11个木薯品种进行遗传基础分析,初步建立了11个木薯品种的AFLP指纹图谱。经PCR扩增,6对引物共获得3560个片段,其中,多态性条带2438个,特异性条带90条,平均多态性条带比率0.68。其遗传相似性系数变化范围在0.578-0.774,多样性水平属中等。通过UPGMA聚类分析表明,在遗传距离0.345处可以将11个木薯品种处分为3类,其中华南8号、华南205和华南6068木薯为一类,GR911和华南124为一类,其他为第三类。
11 cassava varieties from national nursery of cassava germ were amplified by PCR with 6 AFLP primers （ GAA/CAC, GAA/CAG, CAA/CTT, GAG/CTC, GAT/CTC, GAT/CTT） to establish DNA fingerprinting. The results showed that there were total 3560 bands, ineluding 2438 polymorphs bands and 90 special bands. It was 0.68 average polymorphs among the whole subjects. For the genetic similarity, the range was 0. 578 - 0. 774, it was middle level in polymorphism for the all eassava varieties. Meanwhile, the Ntays-pc software was used to cluster the 11 varieties into three types by UPGMA method at the 0. 345 value. SC8, SC205, SC6068 belonged to the first type, the seeond type included GRgl 1 and SC124, and the rest was the third type.
出处
《西南农业学报》
CSCD
北大核心
2010年第5期1606-1609,共4页
Southwest China Journal of Agricultural Sciences
基金
中央级公益性科研院所基本科研业务费专项项目(PZS-007)
现代农业产业技术体系项目(nycytx-17)
