摘要
目的实时定量RT-PCR已被广泛应用于基因的表达分析。选择合适的内参基因可以消除不同标本在RNA的产量、质量以及逆转录效率上可能存在的差别,从而获得目标基因特异性表达的真正差异。方法本研究应用实时定量PCR技术,研究了HMBS、C-TBP、HPRT、UBC和18srRNA5个内参基因在男性乙肝相关肝癌组织中的表达情况。结果结果表明,这5个内参基因表达存在差异。结论经过geNorm程序统计学分析,确定了HMBS和C-TBP两个看家基因适用于校正目标基因的表达量,为研究男性乙肝相关肝癌组织中目标基因的表达奠定基础。
Objective Real-time quantitative RT-PCR had been widely used in gene expression analysis.The selection of suitable endogenous reference genes could be eliminated in the RNA of different samples of the output,quality and RT efficiency differences may exist,which was the real target of specific gene expression differences.Methods In this study,real-time quantitative PCR technique to study the HMBS,C-TBP,HPRT,UBC and 18s rRNA5 one internal reference genes in male HBV expression in hepatocellular carcinoma.Results The results showed that five internal reference gene expression differences.Conclusions After geNorm statistical analysis procedures to determine the HMBS and C-TBP two housekeeping genes suitable for expression of target genes corrected for the study of male hepatitis B-related hepatocellular carcinoma can lay the foundation for the expression of target genes.
出处
《齐齐哈尔医学院学报》
2010年第18期2857-2858,2861,共3页
Journal of Qiqihar Medical University
关键词
实时定量RT-PCR
内参基因
肝癌
HBV
Real-time quantitative RT-PCR Internal reference gene Hepatic carcinoma HBV
