摘要
目的观察山豆根对大鼠神经行为的影响及神经病理的改变，建立一种新的肌张力障碍动物模型。方法雌性SD大鼠24只，按照随机数字表法分为实验组和对照组，每组各12只。实验组大鼠通过灌胃给药法给予中药山豆根煎剂1．5mL，对照组则给予等量的生理盐水，1次／d，连续10d。观察2组大鼠的神经行为变化，并用Noldus动物行为分析系统、转棒实验仪对大鼠运动行为和协调能力进行量化分析，行为实验结束后完整取脑，观察纹状体、中脑和海马的病理形态学变化。结果实验组大鼠在灌注中药山豆根汤剂后3～7d开始出现程度不同的神经行为学症状．主要表现为运动减少，平衡能力下降，颈部及躯干扭转。运动行为轨迹分析显示实验组大鼠运动距离、平均运动速度、直立次数、活动性指标数值均明显小于对照组，运动协调能力明显下降，在转棒上停留的时间较对照组明显缩短，差异均有统计学意义（P〈0．05）。病理学检查显示实验组大鼠纹状体、海马及中脑神经元细胞数与对照组相比有减少趋势，其中以海马部位变化为著。结论中药山豆根对大鼠神经行为的影响与该药中毒造成的人体肌张力障碍表现极为相似，表现为运动障碍，运动协调性差，推测与肌张力障碍有关。病理学上的改变显示这些神经行为的改变有神经病理改变的基础．可作为一种新的肌张力障碍的动物模型。
Objective To investigate the effects of Sophora tonkinensis Gapnep on neural behavior and cerebral pathology of rats. Based on the observation and quantitative analysis of neural behavior, a new type of animal models with dystonia is expected to be demonstrated. Methods Twenty-four female SD rats, weighting 250 g, were equally randomized into control and experimental groups. The animals in the experimental group were treated with 1.5 ml Sophora tonkinensis Gapnep once daily for consecutive 10d. For rats in the control group, the same volume of saline （0.9%） was delivered by the same method. Neurobehavioral changes were observed; and quantitative analysis of neurobehavior and balancing ability was performed by using Noldus and Rota Rod systems. After behavioral experiments, the rats were sacrificed and the brains were got for pathological observations, especially the striatum, midbrain and hippocampus. Results Three to 7d after the treatment, rats in the experimental group presented different degrees of neurobehavioral changes, mainly manifested as hypokinesia, decreased balanced capacity and postural dystonic abnormalities of the neck and limbs. Behavioral analysis showed that the distance, mean velocity and value of activity index in rats of the experimental group were significantly decreased as compared with those in the control group （P〈0.05）. The experimental group showed a more serious dysfunction of the coordinating movement in the tested by rotating rod, a significantly shorter time of staying in the rod as compared with the control group （P〈0.05）. Pathological investigation clearly demonstrated that the experimental group had decreased neurons in the corpus striatum and midbrain, especially in the hippocampus, and some neural pathological findings were observed. Conclusion Sophora tonkinensis Gapnep can induce significant changes of neural behavior in rats, which is similar to the symptoms of patients with dystonia caused by Sophora tonkinensis Gapnep poisoning, mainly manifested as hypokinesia, dysfunction of the coordinating movement, indicating that rats in the experimental group maybe a new type of animal model of dystonia, which is important in the study ofdystonia.
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2010年第10期1027-1032,共6页
Chinese Journal of Neuromedicine
基金
清华-伍舜德医学科研基金（20240000810）
