摘要
目的:研究地塞米松对特发性血小板减少性紫癜(ITP)树突状细胞(DC)免疫活性的影响。方法:36例初诊ITP患者口服地塞米松40 mg/d,连用4 d;分离21例治疗有效患者治疗前后及正常对照外周血单个核细胞,加入细胞因子rhGM-CSF及rhIL-4培养诱导DC,用流式细胞技术检测DC表型;酶联免疫吸附法检测DC培养上清液中IL-12 p70。结果:①ITP患者DC的CD80和CD86阳性表达率分别为(51.60±13.47)%和(61.50±15.93)%,明显高于正常对照的(36.03±15.43)%和(40.28±11.49)%(P<0.05);治疗后ITP患者CD80和CD86表达率分别降为(36.48±15.19)%和(44.05±17.70)%,与治疗前相比,均差异有统计学意义(均P<0.05),但与对照组比较,差异无统计学意义(P>0.05)。②治疗前ITP患者组IL-12 p70为(67.52±14.43)pg/ml,明显高于正常对照的(39.78±10.03)pg/ml(P<0.05);治疗后ITP组IL-12 p70的表达降至(43.90±8.49)pg/ml,与治疗前相比差异有统计学意义(P<0.05),与正常对照相比差异无统计学意义(P>0.05)。结论:DC及其分泌的细胞因子在ITP发病中起重要作用,地塞米松可能通过抑制DC的免疫活性而达到治疗目的。
Objective：To study the effect of dexamethasone（DXM） on the immunoreactivity of dendritic cells in the patients with chronic idiopathic thrombocytopenic purpura.Method：Thirty-six newly diagnosed patients with chronic idiopathic thromobocytopenic purpura received oral high dose（HD） DXM at single daily doses of 40 mg for 4 consecutive days.The peripheral blood mononuclear cells isolated from 21 remission patients and normal controls were stimulated by rhGM-CSF and rhIL-4.The surface antigen of the dendritic cells was analyzed by flow cytometry.The level of IL-12p70 in the supernatant was detected by enzyme linked immuneosorbent assay.Result：In ITP patients,the expressions of both CD80 and CD86 in dendritic cell were significantly higher compared with those in normal controls [（51.60±13.47）% vs（36.03±15.43）%,（61.50±15.93）% vs（40.28±11.49）%,respectively,P0.05].After HD-DXM treatment,both CD80 and CD86 were decreased significantly without difference between HD-DXM treatment patients and normal controls（P0.05）.The level of IL-12p70 in ITP patients was significantly higher [（67.52±14.43） pg/ml] than in controls [（39.78±10.03） pg/ml,P0.05].After the treatment,IL-12p70 decreased significantly to（43.90±8.49） pg/ml with no significant difference between ITP group and control group（P0.05）.Conclusion：Dendritic cells and their cytokine secretion play an important role in ITP,and DXM may achieve its therapeutic effect on ITP by inhibiting the immunoreactivity of dendritic cells.
出处
《临床血液学杂志》
CAS
2010年第5期549-551,共3页
Journal of Clinical Hematology
基金
山东省医药卫生科研项目(No:2007HW014)
山东省烟台市科学技术发展计划项目(No:2007139-9)
