摘要
目的 :探讨乙型肝炎病毒 ( HBV)前 C区基因突变与慢性乙型肝炎中医证型的关系。方法 :将 162例慢性乙型肝炎患者按中医辨证分为 5种证型 ,应用聚合酶链反应 -单链构象多态分析 ( PCR- SSCP)银染技术检测 HBV前 C区基因变异。结果 :慢性乙型肝炎湿热中阻型患者 HBV前 C区突变率为 61.1% ( 3 3 / 5 4 ) ;瘀血阻络型为5 7.6% ( 19/ 3 3 ) ;肝郁脾虚型为 2 9.8% ( 14 / 4 7) ;肝肾阴虚型为 3 8.1% ( 8/ 2 1) ;脾肾阳虚型为 14 .3 % ( 1/ 7)。结论 :HBV前 C区突变与慢性乙型肝炎中医证型密切相关。湿热中阻型和瘀血阻络型突变率明显高于肝郁脾虚型和脾肾阳虚型。
Objective:To study the precore mutation in patients with chronic hepatitis B and its relevence to syndrome′s types of TCM.Methods:1 62 patients with hepatitis B were divided into five typessyndrome of TCM.Single strand conformation polymorphism analysis of polymerase chain reaction product( PCR- SSCP) method was employed to detect hepatitis B virus( HBV) precore mutation in 1 62 cases.Results:the mutation rate was61 .1 % ( 33/5 4) in the group of retention of wetness- heat evil,and5 7.6% ( 1 9/33) in the group of obstruction of blood stasis,2 9. 8% ( 1 4 /4 7) in the group of stagnation of liver- energy and asthenia of spleen,1 4 .3% ( 1 /7) in the group of dificiency of both spleen- yang and kidney- yang and38.1 % ( 8/2 1 ) in group of deficiency of liver- yin and kidney- yin.Conclusion:The mutation of HBV precore gene was relevant to the syndrome types of TCM.The rate of mutation in the group ofretention ofwetness- heatevil and in the group of obstruction of blood stasis was higher than that in the group of stagnation of liver energy and asthenia and the group of dificiency of both spleen- yang and kidney- yang
基金
国家中医药管理局重点课题! ( No.95 4 2 14 9)
