摘要
采用ＴｅｎａｘＧＣ动态吸附法对茶树茶尺蠖Ｅｃｔｒｏｐｉｓｏｂｌｉｑｕａ（Ｐｒｏｕｔ）单白绵绒茧蜂Ａｐａｎｔｅｌｅｓｓｐ．三重营养关系中茶树的新梢挥发性化合物进行了捕集，经ＧＣ、ＧＣＭＳ鉴定结果表明，不同来源的挥发物的组成和数量是不同的，完整茶树新梢（ＩＳ）的挥发物有１１种组份；机械损伤新梢（ＭＤＳ）的挥发物有１３种组份；茶树茶尺蠖取食复合体（ＰＨＣ）的挥发物有１５种组份，而用经典的ＳＤＥ法获得的新梢精油有１３种组份，４种挥发物数量的大小顺序为ＳＤＥ＞ＭＤＳ＞ＰＨＣ＞ＩＳ。生物测定结果表明，无气味经历的雌单白绵绒茧蜂对ＩＳ和ＭＤＳ的气味的选择作用无统计上的显著性，对机械损伤新梢＋幼虫口腔分泌物（ＭＤＳ＋Ｒｅｇ）。
The volatiles emitted from the various treated tea shoots were dynamically entrapped by Tenax GC and identified by GC and GC MS. Results showed that the volatiles emitted from various treated tea shoots differed in composition and relative contents. Volatile emitted from intact tea shoots (IS) was composed of a blend of 11 components; while volatile emitted from mechanically damaged tea shoots (MDS) was a blend of 13 components and volatile from plant host feeding complex (PHC) was a blend of 15 components. Volatile extracted from tea shoots by SDE method was identified to be a blend of 13 components. The order of relative contents from above treatments is SDE>MDS>PHC>IS. Y tube olfactometer bioassay results showed that inexperienced female wasps had no preference to volatiles emitted from IS and MDS over clean air (control) statistically, while strong preference to volatiles emitted from MDS+Reg, LDS, and PHC over control was observed.
出处
《昆虫学报》
CAS
CSCD
北大核心
1999年第2期126-131,共6页
Acta Entomologica Sinica
基金
国家自然科学基金
浙江省自然科学基金
关键词
茶树
茶尺蠖
单白绵绒茧蜂
挥发物
分离
鉴定
tea plant, tea geometrid, Apanteles sp., tritrophic system, volatile
