摘要
本研究采用人工气候室模拟自然热环境对大白鼠进行急性热暴露实验。用苯酚法提取大白鼠肝脏总RNA;用 Oligo(dT)-纤维素亲和层析柱分离出Poly(A)^+mRNA。将各条件下的大白鼠肝脏Poly(A)^+mRNA在麦胚无细胞体外转译系统中表达。结果证明急性热暴露大白鼠肝脏同样能生成分子量分别为71kD、90kD、98kD和 110kD的一组热休克蛋白。
Sixteen Wistar rats were randomly divided into four groups. One group was put at an ambient temperature of 23℃—25℃ as control. The other groups were exposed in an artificial climatic chamber to DB 40℃, WB 32℃, BG 46℃ and Rh 60％. These rats were decapitated after the rectal temperature rose to 41℃, 42℃ and 43℃ respectively for one hour. Isolation of total RNA from control and heat-shocked rat liver by phenol/chloroform/iscamylalcohol method and fractionation of Poly(A)^+mRNA by Oligo (dT)-cellulose chromatography were performed. The difference of Poly(A)^+mRNA between control and heat-shocked rat liver was shown by urea agarose gel electrophoresis. In vitro translation of Poly(A)^+ mRNA in wheat germ system, the synthesis of four major heat shock polypeptides with approximate molecular weights of 71000, 90000, 98000 and 110000 Dalton was observed. The relative amount of major heat shock proteins inereased as the rectal temperature of the heat-exposed rats rose. These results indicated that HSP was also induced when rats, as a whole, were exposed to a simulated hot enviroment.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1990年第1期50-53,49,共5页
Progress In Biochemistry and Biophysics
关键词
热休克蛋白
急性热暴露
肝脏
大鼠
heat shock protein, acute heat exposure rat liver
