摘要
目的优化酶解法提取人参皂苷Rg1的工艺条件。方法采用高效液相色谱法为测定方法,以人参皂苷Rg1提取率为指标,通过正交设计试验进行优选。结果纤维素酶酶解为最优方法,且酶用量、酶解时间、酶解温度对人参皂苷Rg1提取率有显著性影响;提取人参皂苷Rg1的最佳工艺条件为:纤维素酶用量为1.4%,酶解60min,酶解温度为45℃。结论优化后的提取工艺简单、稳定、提取率高。
Objective To optimize the enzymolysis technology of ginsenoside Rg1.Methods Taking ginsenoside Rg1 content as the index,orthogonal design method was used for optimization and HPLC for determination.Results Cellulase enzymolysis was the best extracting process,and enzyme amount,enzymolysis time,and enzymolysis temperature had obvious effect on the extraction of ginsenoside Rg1.The optimum extraction technologies were as follows： cellulase amount was 1.4%,enzymolysis time 60 min,the enzymolysis temperature 45 ℃.Conclusion The optimization extraction technology is simple,steady,and the extracting rate is high.
出处
《中草药》
CAS
CSCD
北大核心
2010年第8期1279-1282,共4页
Chinese Traditional and Herbal Drugs
基金
吉林省中医药管理局中医药科技资助项目(08SYS-102)
