摘要
目的探讨肌肽对溴酸钾诱导小鼠肾损伤的改善作用。方法雄性KM小鼠腹腔注射200 mg/kg溴酸钾诱发小鼠肾损伤模型,观察肌肽给药后的改善作用。采用HPLC测定小鼠血清中尿素氮(BUN)、肌酐含量及肾组织中谷胱甘肽(GSH)含量,TBARS法测定小鼠肾组织中丙二醛(MDA)含量和G riess反应法测定血浆及肾组织的一氧化氮(NO)含量,荧光酶标仪检测肌肽的体外及小鼠肾组织的抗氧化能力指数。结果与肾损伤模型组相比,肌肽可以有效降低血清尿素氮、肌酐含量和肾组织中丙二醛含量和一氧化氮水平,提高小鼠肾组织的谷胱甘肽水平及抗氧化能力指数,但肌肽在体外实验中没有显示明显的抗氧化能力。结论肌肽对溴酸钾诱发的小鼠肾损伤具有一定的保护作用,其作用机制不是来自肌肽的直接抗氧化作用,而是可能与缓解溴酸钾诱发的肾组织内的过氧化应激状态有关。
Objective To investigate the protective effect of carnosine on potassium bromate（KBrO3）-induced kidney damage in mice.Methods The kidney damage model was established by receiving a single intraperitoneal injection of 200 mg/kg KBrO3 in male Kunming mice.Blood urinary nitrogen（BUN）,serum creatinine（SCr） and glutathione（GSH） in kidney were measured by high performance liquid chromatography（HPLC）.Malondialdehyde（MDA） in kidney was measured by TBARS method.The content of NO in plasma and kidney were measued by Griess method.Antioxidative activity of carnosine in vitro and in vivo were evaluated by automated multifunctional microplate reader.Results Compared with the model group,carnosine could effectively decrease BUN and SCr levels and the contents of MDA in kidneys,obviously increase the ORAC activity and the content of GSH in kidneys.However,carnosine showed no antioxidative effect in vitro.Conclusion Our findings revealed the protective effect of carnosine on KBrO3-induced kidney damage in mice.The mechanism was related with its effects on improving the oxidative stress induced by KBrO3,rather than its direct anti-oxidative activity in kidney.
出处
《今日药学》
CAS
2010年第8期14-17,共4页
Pharmacy Today
关键词
肌肽
溴酸钾
肾损伤
氧化应激
carnosine
KBrO3
kidney damage
oxidative stress
