摘要
干燥绞股蓝粉碎后用乙醇反复浸泡,合并浸泡液减压浓缩后多次反复用正相硅胶柱层析、反相硅胶(ODS-AQ)柱层析和凝胶LH-20柱层析进行分离,得3个纯化合物Ⅰ、Ⅱ、Ⅲ并对其体外抗氧化和抑菌作用进行研究。结果表明:结构鉴定表明3个化合物均为木脂素类物质。化合物Ⅲ对DPPH自由基有较强清除作用,化合物Ⅰ的清除能力较弱,而化合物Ⅱ在实验浓度范围内没有显示出清除DPPH自由基作用;3种化合物对金属的螯合能力都比较弱;化合物Ⅱ对变异链球菌有一定的抑制作用,对金黄色葡萄球菌有较强的抑制作用,而对大肠杆菌和沙门氏菌显示较弱的抑制作用;化合物Ⅲ对金黄色葡萄球菌、大肠杆菌和沙门氏菌仅显示出微弱的抑制作用,化合物Ⅰ也显示出与化合物Ⅲ相似的抑菌趋势,但作用更弱。
Chemical investigations of the EtOH extract of Gynostemma pentaphyllum （Cucurbitaceae） led to the isolation of 3 lignans, named compounds Ⅰ, Ⅱ and Ⅲ. Compound Ⅲ showed stronger DPPH free radical scavenging activity when compared to BHT at the same concentrations, while compound Ⅰ had only very little DPPH free radical scavenging activity and compound Ⅱ failed to scavenge DPPH free radicals within the experimental concentration range. All the compounds had a weaker ability to chelate Fe2＋ when compared to EDTA. Compound Ⅱ inhibited Streptococcus mutans to a certain extent and strongly inhibited Staphylococcus aureus, while had very little inhibitory effect against Salmonella enteritidis and Escherichia coli; also, compound Ⅲ could weakly inhibit Staphylococcus aureus, Salmonella enteritidis and Escherichia coli; and compound Ⅰ had the same but weaker antimicrobial spectrum as that of compound Ⅲ.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第13期154-157,共4页
Food Science
基金
山西省回国留学人员科研项目(2009040)
关键词
绞股蓝
木脂素
抗氧化
抑菌
Gynostemma pentaphyllum
liganins
antioxidant activity
anti-microbial activity
