摘要
目的探讨As2O3联合L-OHP对HepG2肝癌细胞株的体外抑制作用。方法采用MTT法(四唑盐比色法)动态观察As2O3联合L-OHP对HepG2肝癌细胞的生长抑制作用,并采用两药相互作用系数(CDI)来评价两药相互作用的性质;应用流式细胞术(FCM)检测凋亡率和细胞周期分布。结果 As2O3与L-OHP联合应用,对HepG2肝癌细胞株的生长抑制、诱导凋亡作用均较相应的单药增强。流式细胞直方图上可见明显的"凋亡峰",且As2O3使肝癌细胞周期阻滞于G2/M期,L-OHP使细胞周期阻滞于S期和G2/M期,两药合用使细胞周期阻滞于S期和G2/M期。结论中低浓度As2O3与L-OHP联合作用具有显著的协同效益,其主要机制可能是As2O3联合L-OHP诱导肝癌细胞凋亡作用及增强细胞周期阻滞作用。
Objective To explore the inhibiting effect of Arsenic trioxide（As2O3）combined with Oxaliplantin（L-OHP） on hepatocellular carcinoma cell line HepG2.Methods The inhibiting effect on the cells growth was observed by MTT assay.The character of the two drugs interaction was estimated by coefficient of drug in interaction（CDI）.The cell cycle and apoptotic changes were studied by the flow cytometry（FCM）.Results The inhibiting and apoptosis inducing effect were significantly higher by the combination of As2O3 and L-OHP than by any drug used alone.The cell cycle of HepG2 cells was arrested in G2/M phase by As2O3,in S＋G2/M by L-OHP,and in S＋G2/M phase by As2O3 combined with L-OHP.Conclusion As2O3 combined with L-OHP in moderate-low concentration have synergistic effect in inhibiting the cell growth of HepG2 cell line possibly by inducing carcinoma cell apoptosis and enhancing the function of cell cycle arrest.
出处
《实用癌症杂志》
2010年第4期333-336,共4页
The Practical Journal of Cancer
关键词
三氧化二砷
奥沙利铂
肝癌
细胞株
凋亡
As2O3
L-OHP
Hepatocellular carcinoma
Cell line
Apoptosis
