摘要
为研究和探讨抗肿瘤药物丝裂霉素(mitomycin C,MMC)对巨噬细胞的作用,MMC能否增强巨噬细胞杀伤肿瘤细胞的作用以及与免疫活化剂厌氧棒状杆菌菌苗(CP)的共同作用,本实验将不同剂量MMC,CP,CP+MMC分别注入小鼠腹腔内,收集其中的巨噬细胞,并与肺癌细胞(A549)混合培养,运用相差显微镜、光镜及显微分光光度计等方法进行观察和检测。发现MMC对巨噬细胞具有活化作用,受其作用的A549胞浆内空泡及颗粒增多、边界不清。不同实验组巨噬细胞计数提示,各MMC组较对照组有不同程度减少,CP组明显增加,CP+MMC组增加更为明显。DNA相对含量测定发现对照组及各实验组间巨噬细胞DNA量无显著差异(P>0.05)。而活化的巨噬细胞与A549作用后,A549 DNA的曲线明显左移,DNA量较少的细胞增多,与对照组相比有显著差异(P<0.05),但各实验组之间无明显差异(P>0.05)。由此可见,MMC对巨噬细胞杀伤肿瘤作用具有一定的影响。
In oreer to investigate the action of antitumor agent mitomycin C (MMC) on macrophages, the effect of MMC on the tumoricidal action of macrophages and the action of MMC used in combination with the immunostimulant, i.e. corynebacterium parvum, 3 groups of CFW mice were given i.p. injection of different doses of MMC (2, 3, 4, 5 mg/kg), CP (1mg/mouse) and CP+MMC. The adherent macrophages were isolated from peritoneal exudate cells 4 days after injection. Peritoneal macro- phages were cocultured with A 549 (a tumor cell line from human alveolar cell carcinoma of lung) in E/T 10:1 for 24 hours and studied with phase contrast microscopy, conventional light microscopy (HE stain and Feulgen stain) and the relative DNA content of macrophage and A 549 were measured with microspectrophotometer. We found that MMC could activate mouse peritoneal macrophages. When A 549 cells were cocultured with MMC-activated macrophages, an increase in the number of vacules and granules was found in the cytoplasm of A549 cells. The number of macrophages in MMC group Was lower than that of the control group, the number of macrophages in CP group was higher than that of the control group and the number of macrophages in CP+MMC group was markedly increased in contrast with that of the CP group. Determination of the relative content of DNA macrophages and A549 revealed that there were no significant differences in DNA content of macrophages between the control group and the experimental group (P>0.05).But when A549 cells were cocultured with activated macrophages, the DNA content of A549 cells decreased and the number of A549 cells with less DNA content increased. The difference was significant as compared with the control (P<0.05). But there were no significant differences between any two of MMC group, CP group and CP+MMC group.
出处
《上海第二医科大学学报》
CSCD
1990年第1期23-27,共5页
Acta Universitatis Medicinalis Secondae Shanghai
关键词
丝裂霉素C
巨噬细胞
肿瘤细胞
mitomycin C corynebacterium parvum macrophage
