摘要
目的探讨海洛因依赖对大鼠垂体远侧部促肾上腺皮质激素细胞表达促肾上腺皮质激素(ACTH)的影响以及血清皮质醇(COR)的改变,并探讨引起变化的可能机制。方法成年雄性SD大鼠55只,随机分为正常对照组、盐水对照组及海洛因依赖组。皮下注射海洛因,建立海洛因依赖大鼠模型,分别于模型建立的第10、17、24、31、38天取垂体组织。应用免疫组织化学SABC法、图像分析法及放射免疫方法进行研究。结果与正常对照组和盐水对照组比较,海洛因依赖组大鼠垂体远侧部ACTH阳性细胞免疫反应明显减弱,与正常及盐水对照组比较有显著性差异;图像分析显示,海洛因依赖组ACTH阳性细胞的平均灰度值增高(P<0.05〉。放射免疫法测定结果显示,海洛因依赖组血清COR含量较正常对照组明显降低,经统计处理,差异有统计学意义(P<0.05)。结论在海洛因依赖期间,垂体远侧部ACTH阳性细胞表达减少,血清COR含量降低,提示在大鼠海洛因依赖期间,垂体-肾上腺轴功能受到抑制。
Objective To investigate the effect of heroin dependence on the expression of adrenocorticotrophic hormone （ACTH） in ACTH positive cells of pars distalis hypophyseos and the level of serum cortisol for exploring the possible mechanism of the changes. Methods Fifty-five adult male SD rats were randomly divided into a normal control group （NCG）,a saline control group （SCG） and a heroin dependence group （HDG）. Heroin-dependent rat models were set up by the way of subcutaneous injection of heroin and the hypophysis were excised on the 10th, 17th, 24th, 31st and 38th days after models were set up. Immunohistochemical SABC method, imagine analysis and radioimmunoassay （RIA） were used in the research. Results Compared with the NCG and SCG, the immune staining intensity of ACTH cells in HGD was weakened. The average gray value was significantly higher （P 〈 0. 05）. The result of RIA showed that the level of serum eortisol in HDG decreased as compared with the NCG（P 〈 0.05）. Conclusions During heroin dependence, the expression of ACTH positive cells diminished, the level of serum cortisol reduced. The morphological and functional changes suggest that the heroin inhibits the function of the pituitary-adrenal axis.
出处
《解剖学报》
CAS
CSCD
北大核心
2010年第3期349-352,共4页
Acta Anatomica Sinica
基金
贵州省优秀科技人才省长基金(2005)14号
高层次人才基金(2007)26号资助
