摘要
目的探讨多药耐药基因（ｍｄｒ１基因）在人胃癌组织中的表达及其与临床病理的关系．方法应用ＲＴＰＣＲ法检测了２９例手术切除人胃癌组织中的ｍｄｒ１ｍＲＮＡ．术前未用过化疗．结果ｍｄｒ１ｍＲＮＡ的阳性率为４８３％（１４／２９），ｍｄｒ１ｍＲＮＡ的表达在肿瘤浸润浆膜者为３３３％（７／２１），明显低于未浸润浆膜者（７／８），基因的表达与年龄、性别、肿瘤大小、组织学类型、淋巴结转移及ＴＮＭ分期等无关．结论化疗前胃癌组织中ｍｄｒ１基因即存在较高的表达率，这为选用化疗药物和ＭＤＲ逆转剂提供了参考指标．ｍｄｒ１ｍＲＮＡ表达减少似与肿瘤进展相关．
AIM To explore the relationship between expression of mdr1 gene in human gastric cancer and clinicopathological changes. METHODS From Sept 1997 to Jan 1998, a total of 29 patients with gastric carcinoma were recruited, and mdr1 mRNA were assayed by RT PCR. Fresh tumor tissues were surgically resected without chemotherapy before operation. RESULTS The positive rate of mdr1 mRNA was 48 3% (14/29). The expression rate in patients with serosa invasion was 33 3% (7/21), much lower than in those without serosa invasion (87 5%). The correlation between mdr1 expression and the age and sex of the patients, tumor size, histologic types, lymph node involvement and TNM stage was not significant. CONCLUSION There are intrinsic drug resistance in almost half of the primary gastric carcinomas prior to chemotherapy. Analysis of mdr1 gene can be helpful in selecting chemotherapeutic drugs and mdr1 mRNA may act as a reference index for using the MDR reversing agents. The absence of mdr1 mRNA expression seems to correlate with advanced disease, which needs to be further confirmed.
出处
《世界华人消化杂志》
CAS
1999年第2期145-146,共2页
World Chinese Journal of Digestology
关键词
胃肿瘤
多药耐受性
MDR1基因
基因表达
stomach neoplasms
resistance, multiple drugs
mdr1 gene
RNA, messenger
gene expression
