摘要
目的在人类幽门螺杆菌(Hp)感染的C57BL/6小鼠模型中,研究中性粒细胞激活蛋白C-末端区域疫苗治疗Hp感染的作用。方法把已感染Hp的C57BL/6小鼠分成4组,分别通过灌胃法给予单纯中性粒细胞激活蛋白C-末端蛋白(100μg)、中性粒细胞激活蛋白C-末端蛋白(100μg)加霍乱毒素(CT,2μg)、单纯CT(2μg)或PBS,每周1次,共4次,治疗4周后处死动物,取胃黏膜行半定量细菌培养检查Hp情况。结果治疗后各组Hp根除率分别为:中性粒细胞激活蛋白C-末端蛋白加CT组50%(5/10),单纯中性粒细胞激活蛋白C-末端蛋白组、单纯CT组及PBS组根除率均为0%。统计分析表明中性粒细胞激活蛋白C-末端蛋白加CT组的H.pylori根除率较其它组有显著性差异(P<0.05)。此外,未根除Hp的小鼠,中性粒细胞激活蛋白C-末端蛋白加CT组Hp的定植密度明显低于其它3组(P<0.05)。结论中性粒细胞激活蛋白C-末端蛋白能够作为Hp多价治疗疫苗的组分之一。
Aim To investigate the effects of the vaccine consisting of Helicobacter pylori（H.pylori）C-terminal region of neutrophil activated protein and cholera toxin（CT）in the treatment of H.pylori infection in a H.pylori infected mouse model.Methods C57BL/6 mice infected with H.pylori were divided into four groups.The mice of each group were immunized by C-terminal region of neutrophil activated protein（100μg）plus CT（2μg）,C-terminal region of neutrophil activated protein（100μg）,CT（2μg）,PBS orally respectively once a week for four weeks.Four weeks after last treatment,all animals were sacrificed and stomach biopsies were collected to detect H.pylori by semi-quantitative bacterial culture assay.Results The eradication rate of the vaccine group was 50%（5/10）.The eradication rate of the other three groups was all zero.There were significantly differences in the eradication rate among vaccination group and the other three groups（P〈0.05）.In addition,the colonized H.pylori density in vaccination group was significantly lower than that of other three groups（P〈0.05）.Conclusion C-terminal region of neutrophil activated protein could be one of components of H.pylori multivalent vaccine.
出处
《现代消化及介入诊疗》
2010年第2期63-65,共3页
Modern Interventional Diagnosis and Treatment in Gastroenterology
