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转化生长因子β_1对子宫内膜癌细胞系Ishikawa体外增殖、细胞周期、凋亡的影响 被引量：4

Effect of TGF-β_1 in Proliferation,Cell Cycle and Apoptosis of the Endometrial Carcinoma Cell Line Ishikawa in vitro
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摘要 目的探讨转化生长因子β(1TGF-β1)对子宫内膜癌细胞系Ishikawa体外增殖、细胞周期及凋亡的影响。方法用MTT法检测细胞增殖水平,通过流式细胞术观察细胞周期和凋亡的变化。结果TGF-β1以时间依赖方式明显抑制Ishikawa细胞的生长(P<0.05),各浓度间差异显著,随浓度增高抑制更加显著(P<0.05)。TGF-β1促进Ishikawa细胞凋亡,细胞凋亡百分比随时间及浓度的增加而增长;TGF-β1阻滞细胞停滞于G1期,S期的比例显著降低。结论TGF-β1能抑制人子宫内膜癌Ishikawa细胞的增殖,促进细胞凋亡,阻滞细胞周期。 Objective To investigate the effect of TGF-β1 in proliferation,cell cycle and apoptosis of the endometrial carcinoma cell line Ishikawa in vitro.Methods MTT assay was used to measure the proliferation of cells at different time and different concentration.Flow cytometry was employed to investigate cell apoptosis and cell cycle.Results TGF-β1 inhibited the proliferation of Ishikawa cell,and it had significant difference at different concentration of TGF-β1（P〈 0.05）.TGF-β1 could promote the apoptosis of Ishikawa cell.TGF-β1 apparently blocked the Ishikawa cell at G1 phase.Conclusion TGF-β1 may partly inhibit the proliferation of Ishikawa cell,promote its apoptosis and block the Ishikawa cell at G1 phase.
作者 李莹莹 刘贵鹏 王东娇 张璐
机构地区 中国医科大学附属盛京医院妇产科 辽阳市第三人民医院妇产科
出处 《中国医科大学学报》 CAS CSCD 北大核心 2010年第4期255-257,共3页 Journal of China Medical University
基金 辽宁省教育厅科学研究计划项目(05L474)
关键词 转化生长因子β1 人子宫内膜癌细胞 ISHIKAWA 凋亡 细胞周期 TGF-β1 human endometrial carcinoma cell Ishikawa apoptosis cell phase
分类号 R737 [医药卫生—肿瘤]
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参考文献9

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同被引文献37

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中国医科大学学报
2010年 第4期

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