摘要
目的研究环氧合酶-2(COX-2)在重症急性胰腺炎(SAP)大鼠肝组织中的表达,探讨COX-2在SAP肝脏损伤中的作用。方法以3.5%牛磺胆酸钠胰胆管逆行注射诱导大鼠SAP模型。随机分为对照组和SAP组,术后4、8、16及24h测定血清淀粉酶(AMY)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、肿瘤坏死因子(TNF)-α水平和腹水AMY;光镜下观察胰腺和肝组织损伤情况;免疫组化方法检测COX-2在肝组织中的表达。结果SAP组各时间点血清AMY、ALT、AST、TNF-α及腹水AMY水平均较对照组明显升高,差异均有统计学意义(P<0.05),与胰腺和肝脏组织的病理学改变相一致。术后不同时相COX-2在肝组织中的表达阳性率明显高于对照组,差异均有统计学意义(P<0.05),COX-2表达与ALT(rs=0.949,P=0.039)、AST(rs=0.972,P=0.016)及血清AMY(rs=0.944,P=0.041)呈正相关。结论SAP时COX-2表达上调可能在SAP合并肝脏损伤中发挥重要作用。
Objective To investigate the expression of cyclooxygenase-2 （COX-2） in liver tissue of severe acute pancreatitis （SAP） rats.Methods A rat model of SAP was induced by retrograde infusion of 3.5% sodium taurocholate into the biliary-pancreatic duct.Eighty rats were randomly divided into SAP group and control group.The levels of serum amylase （AMY）,alanine aminotransferase （ALT）,aspartate aminotransferase （AST）,tumor necrosis factor （TNF）-α and ascites AMY were detected dynamically at 4 h,8 h,16 h and 24 h after operation.The pancreatic and liver injuries were observed by light microscope.The expression of COX-2 in liver tissue was measured by immunohistochemistry.Results Compared with the control group,the levels of serum AMY,ALT,AST,TNF-α and ascites AMY increased significantly at the time point of 4 h,8 h,16 h and 24 h （P〈0.05）.These changes were paralleled with the histopathological changes of pancreatic and liver tissue.The expression positive rates of COX-2 at the different time in the SAP group were higer than those of the control group （P〈0.05）.There was a significantly positive correlation between the expression of COX-2 and ALT （rs=0.949,P=0.039）,AST （rs=0.972,P=0.016） and serum AMY （rs=0.944,P=0.041）,respectively.Conclusion Overexpression of COX-2 may play an important role in liver injury during SAP.
出处
《中国普外基础与临床杂志》
CAS
2010年第4期343-347,共5页
Chinese Journal of Bases and Clinics In General Surgery
