摘要
[目的]对不同贮藏条件下金银花花粉生活力进行测定,以期为金银花生产、科研提供参考。[方法]以金银花新鲜花粉为试材,通过I-KI染色法、TTC(氯化三苯基四氮唑)染色法、培养基萌发试验法对花粉生活力进行测定,并研究了不同贮藏条件对花粉萌发的影响。[结果]培养基中添加不同浓度的蔗糖和硼酸对花粉的萌发有促进作用,其浓度分别为150、30mg/L时花粉的萌发率最大值为75.23%和52.78%;I-KI染色法不宜用于金银花花粉生活力测定;采用TTC染色法测定金银花花粉生活力达80.46%。[结论]TTC染色法测定金银花花粉生活力相对准确且简便易行。在金银花花粉贮藏过程中低温贮藏(-80℃)好于冷藏(4℃、-23℃),不宜常温贮藏。
[Objective] Pollen viability of honeysuckle was determined at different storage conditions,which provided a reference for producing and researching of honeysuckle.[Method] Taken the fresh pollen of honeysuckle as an experimental material,I-KI staining,TTC staining and culture medium germination methods were used to determine its fresh pollen ability.Effects of different storage on the pollen germination were studied.[Result] The results showed that addition different concentration of sucrose and boric acid to culture media had promoted effects on the pollen germination.When the concentrations were 150 and 30 mg/L,the germination rate reached the maximum,which were 75.23% and 52.78%.I-KI staining was not suitable for the determination of pollen viability of honeysuckle,while TTC staining was suitable for it and rates reached 80.46%.[Conclusion] This method is relative correct and simple.It was better mid-low temperature storage（-80 ℃）than cold storage（4 ℃、-23 ℃）,it was not suitable for the normal condition.
出处
《安徽农业科学》
CAS
北大核心
2010年第11期5854-5856,共3页
Journal of Anhui Agricultural Sciences
基金
河南省创新人才工程(2005-126-49)
河南科技学院博士基金(050106)
河南科技学院重点项目基金(050121)
关键词
金银花
花粉
生活力
贮藏
Honeysuckle
Pollen
Viability
Storage
