摘要
3年来的主要进展为：完成了重组谷胱甘肽－S－转移酶（GST）免疫兔、牛的免疫持效观察．在国内外首先进行GST基因和K99基因共表达成功．表达产物分子量50kDa，以纤毛形成存在于细菌表面，并分别具有GST和K99抗原活性，为重组GST抗原与幼畜腹泻疫苗在血吸虫病疫区联合应用打下基础．此外，还首次证明日本血吸虫GST与肝片吸虫（Fh）、猪蛔虫（As）有明星交叉保护性免疫力，并完成FhGST和AsGST基因的克隆测序及其与日本血吸虫GST基因同源性分析，对拓宽GST抗原的应用范围及价值有重要意义．天然TPI，Tropomyosin，Paramyosin免疫小鼠试验证明有明显免疫原性后，编码上述3个候选抗原基因均已克隆测序，部分表达或正在进行全基因表达的研究．血吸虫Tropomyosin的研究进展，首次证明该抗原有较强的免疫原性，可选择作为日本血吸虫基因工程混合疫苗的候选抗原之一．
The observation on persistency of the immunity induced by recombinant 26kDa GST of Schistosoma japonicum was carried out in rabbit experiments and cattle field trials. Main success has been made in the co-expression of the genes encoding Sj26GST and K99. The co-expressed products were assayed by ELISA, IHA, transmission electron microsopy, SDSPAGE, Western blot etc. Co-expressed products can react with anti-GST and anti-K99 antibodies, and act as pilus on the surface of E. coli which is 50kDa in molecular weight. Thisstudy will play an important role in combination of schistosome vaccine with vaccine againstfatal enterotoxigenic E. coli or entricolibacillosis in endemic area of schistosomiasis. The protective efficacy of GST from Faciola hepatica and Ascaris suum was first evaluated anddemonstrated in mice against challenge infection with cercariae of S. japonicum. Cloning, sequencing and homology analysis of cDNA encoding FhGST and AsGST were studied and elucidated. The vaccination experiments in mice immunized with native TPI, tropomyosin,paramyosin were completed. The results showed that all of above vaccine candidates have obvious immunogenecity. Molecular cloning and sequencing of above 3 vaccine candidates havebeen carried out. Partial expression or entire cDNA expression of genes encoding above candidate antigens are under investigation. Tropomyosin can be served as a new candidate antigenof vaccine against S. japonicum because it can induce strong immune response not only due toits antigenicity but also immunogenicity against S. japonicum infection.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
1998年第A07期78-78,共1页
Chinese Journal of Schistosomiasis Control
基金
总理预备金血吸虫疫苗研究项目!94-Y-45
关键词
日本血吸虫
重组抗原
疫苗
基因工程
Schistosoma japonicum, recombinant antigen, vaccine, glutathione-s-transferase,triosephosphate isomerase, paramyosin, tropomyosin
