摘要
目的探讨槲皮素诱导HepG2细胞凋亡的作用机制。方法应用透射电镜观察药物作用组及对照组细胞形态学变化;AnnexinV荧光染色和流式细胞仪检测药物作用组及对照组HepG2细胞凋亡和死亡率,以及逆转录聚合酶链反应(RT-PCR)法检测药物作用组及对照组凋亡相关基因fas的变化。结果槲皮素对HepG2细胞的生长有明显的抑制作用,并诱导肿瘤细胞发生凋亡,凋亡细胞表现为细胞固缩、核染色质碎裂,流式细胞仪检测凋亡率为13.2%,细胞停在G1和G2期。AnnexinV标记的方法检测凋亡时发现,坏死与凋亡共存。在槲皮素诱导HepG2细胞凋亡过程中,凋亡相关基因fas转录水平比用药前增强。结论诱导凋亡为槲皮素抑癌的机制之一,槲皮素诱导HepG2细胞凋亡可能与fas基因表达有关。
Objective To study the mechanism of quercetin - induced apoptosis of human HepG2 cells. Methods The cellular morphology of each research group were observed by transmission electron microscope ; the cells were staining by Annexin V and the flow cytometry （FCM） were applied to detect apoptosis of the HepG2 cells ; and the reverse transcription polymerase chain reaction （RT- PCR） were applied to detect the genes related with apoptosis, such as fas. Results Quercetin had a evident effect on the growth of the human liver cancer HepG2 cells as well as apoptosis inducing. The morphous of HepG2 cells showed nuclear chromatine condensation and nuclear fragmentation. The apoptosis rate of HepG2 cell was 13.2% by FCM technolony and the cell cycle stopped at the G1 and G2 phase. The transcription level of the fas gene was enhanced. Conclusion Quercetin could induce apoptosis of the human HepG2 cells as a inhibitor of liver cancer. The mechanism of induction apoptosis may be related to the fas gene activation.
出处
《实用心脑肺血管病杂志》
2010年第3期310-311,共2页
Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease
