摘要
马绒毛膜促性腺激素(equine chorionic gonadotropin,eCG)是由妊娠期母马胎儿尿膜—绒毛膜细胞分泌的一种由α、β两个亚基组成的异源二聚体糖蛋白,具有FSH、LH的双重活性。采用PCR-SSCP和测序方法对28匹蒙古马和25匹纯血马的eCG/LHβ亚基基因的多态性进行研究,结果发现,在蒙古马eCG/LHβ亚基基因5′调控区存在一突变位点:G-415A分为AA、BB、AB 3种基因型,AA、BB、AB基因型频率分别为0.89、0.07、0.04;纯血马此基因中未检测到多态性。
28 Mongolia mares and 25 Thoroughbred mares were subjected to SNP analysis by PCR-SSCP and sequencing,one SNPs was found in 5′flanking region of eCG/LHβ gene,the mutation G-415A was found in Mongolia mares but not in Thoroughbred mares,the frequent of three genotypes：AA,BB,AB are 0.89,0.07,0.04,respectively.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第2期111-114,共4页
China Animal Husbandry & Veterinary Medicine
