摘要
研究胶束在线扫集毛细管电泳法测定三聚氰胺的可行性,结果表明,与区带毛细管电泳相比,胶束在线扫集毛细管电泳法富集倍数提高约60倍。缓冲体系为140 mmol/L SDS＋20 mmol/L NaH2PO4（pH 2.20）＋10%（体积分数）甲醇,分离电压-18 kV,进样时间30 s,测量波长214 nm。考察了SDS浓度、pH、进样时间、运行电压等因素对分离测定的影响情况。在优化条件下,三聚氰胺在9 min时出峰,峰面积RSD≤3.7%。方法检出限、线性范围、相关系数分别为：0.13μg/mL、0.50～32.0μg/mL、0.9997。方法可用于奶粉中三聚氰胺的分离测定。
On-line sweeping micellar electrokinetic chromatography（sweeping-MEKC） and capillary zone electrophoresis（CZE） were used for the determination of melamine.Compared to CZE,the sensitivity was improved about 60 times by sweeping-MEKC.The buffer medium contains 140 mmol/L SDS＋20 mmol/L NaH2PO4（pH 2.20）＋10% methanol,separation voltage was-18 kV,sample injection time was 30 s,and the samples were detected at 214 nm.The effect of SDS concentration,pH in background solution,sample injection time and applied voltage were optimized. Under the optimum conditions, the determination time was within 9 min with RSD of peak area less than 3.7%. The LOD was 0.13 μg/mL, calibration plot was linear in the range of 0.50- 32.0μg/mL with r ≥0.9997. Fuahemlore, this proposed sweeping-MEKC method was applied to the detection of melamine in powdered milk with satisfactory results.
出处
《分析试验室》
CAS
CSCD
北大核心
2010年第1期76-79,共4页
Chinese Journal of Analysis Laboratory
关键词
毛细管电泳
胶束扫集
三聚氰胺
Capillary electrophoresis
Sweeping micellar electrokinetic chromatography
Melamine
