摘要
本文对大陆株日本血吸虫２６ｋＤａ谷胱甘肽Ｓ－转移酶（Ｓｊ２６ＧＳＴ）的ＤＮＡ疫苗进行研究，并观察了其免疫保护效果。分别构建含Ｓｊ２６ＧＳＴ基因的重组质粒ｐＣＤ－Ｓｊ２６和ｐＢＫ－Ｓｊ２６，采用脂质体介导的基因转移将ｐＣＤ－Ｓｊ２６和ｐＢＫ－Ｓｊ２６分别导入ＮＩＨ３Ｔ３成纤维细胞，用免疫荧光法（ＩＦＡ）证实Ｓｊ２６ＧＳＴ在真核细胞中的表达。分别将ｐＣＤ－Ｓｊ２６和ｐＢＫ－Ｓｊ２６及ｐＢＫ－ＣＭＶ肌注ＢＡＬＢ／ｃ小鼠。免疫３次后，免疫鼠产生一定水平的抗血吸虫抗体。用日本血吸虫尾蚴攻击感染免疫小鼠，结果ｐＣＤ－Ｓｊ２６和ｐＢＫ－Ｓｊ２６免疫鼠减虫率分别为３７．１９％和４４．４４％，肝组织减卵率为７３．８０％和４７．２０％，每对成虫产卵减少５６．８８％和１５．６７％。实验结果表明，日本血吸虫Ｓｊ２６ＤＮＡ疫苗免疫小鼠后能诱生一定水平的抗血吸虫抗体，免疫鼠可形成一定水平的保护性免疫力，预防血吸虫尾蚴感染。
DNA vaccines, the antigenencoding DNAs used in vaccination, represent a novel approach to the development of subunit vaccines. In this study,the plasmid pCDSj26 and pBKSj26,both of which contain the fulllength cDNA of 26 kDa glutathione Stransferase of S. japonicum(Sj26GST)were constructed. The pCDSj26 and pBKSj26 were transiently transfected into NIH3T3 cells. The expression of Sj26GST in transfected cells was confirmed by indirect immunofluoresent assay. The BALB/c mice were inoculated by intramuscular injection with pCDSj26,pBKSj26 and pBKCMV(control plasmid), respectively. Mice immunized with DNA vaccine developed a certain level neutralizing antibody to S. japonicum. Vaccination with pCDSj26 and pBKSj26 resulted in significant protection with 37.19% and 44.44% reduction in worm burden,73.80% and 42.20% reduction in liver tissue eggs, 56.88% and 15.67% reduction in female worm fecundity, respectively. The results indicate that Sj26 GST DNA vaccine can induce a certain level of specific protective immunity in mice against infection with S. japonicum and an antifecundity, theredy reducing pathology.
出处
《中国寄生虫病防治杂志》
CSCD
1998年第3期207-211,共5页
Chinese Journal of Parasitic Disease Control
基金
总理基金
关键词
日本血吸虫
DNA疫苗
保护性免疫
Sj26GST
Schistosoma japonicum
DNA vaccine
glutathione Stransferase
expression
protective immunity
