摘要
[目的]探究2,4-D和6-BA对籼稻成熟胚愈伤组织培养的影响。[方法]在愈伤组织诱导和分化培养基中设置不同的2,4-D和6-BA浓度,研究2,4-D和6-BA对籼稻成熟胚愈伤组织诱导、成苗及再生幼苗生根的影响。[结果]在含0.5 mg/L2,4-D的培养基中,嘉育948、盐恢559、扬籼6547、中二软占、明恢86、广恢998、遵籼3号等7个品种的愈伤组织诱导效果最佳;在含最适2,4-D浓度的诱导培养基中再添加0.2 mg/L6-BA对愈伤组织诱导率作用不明显,但抑制愈伤组织分化成苗;在分化培养基中适当降低6-BA浓度,既能保证愈伤组织成苗率不下降甚至上升,又可以提高再生植株的质量。[结论]该研究结果为2,4-D和6-BA在籼稻愈伤组织培养中的合理使用提供借鉴。
[ Objective ] The study aimed to explore the effects of 2,4-D and 6-BA on indica rice callus culture using mature embryos as explants. [ Method] 2,4-D and 6-BA were set at different concentrations in callus initiation media and plantlet regeneration media in order to study their effects on callus initiation, plantlet regeneration and root initiation. [ Result] The calli of seven varieties, including Jiayu 948, Yanghui 559, Yangxian 6547, Zhongerruanzhan, Minghui 86, Guanghui 998, and Zunxian 3, were best initiated on callus initiation media containing 2,4-D at 0.5 mg/L ; 0.2 mg/L 6-BA was supplemented into callus initiation media containing optimal 2,4-D concentration with an objective to explore the effects of 6-BA on callus initiation and plantlet regeneration, and it was found that the 6-BA treatment had no significant effects on callus initiation frequency, but could reduce plantlet regeneration frequency; not only plantlet regeneration frequency could be maintained even increased, but also the quality of regenerated plantlets could be improved when the concentration of 6-BA in plantlet regeneration medium was properly reduced. [ Conclusion] The results of this study could provide references for reasonable uses of 2,4-D and 6-BA in indica rice callus culture.
出处
《安徽农业科学》
CAS
北大核心
2009年第27期12927-12929,共3页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金资助项目(30571049)
